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1、免疫抑制劑對小鼠腹腔巨噬細(xì)胞的影響論文.freelg/只，環(huán)磷酰胺2.0mg/只.freelethasoneandcyclphosphamideonperitonealmacrophageofmice【Abstract】Objective)inducedbyinjectionofdexamethasoneandcyclophosphamideinmice.MethodsTheimmunefunctionbydeterminingthephagocyticfunctionandstructureofthemacrophagocytesoftheabdomina
2、lcavitiesofmiceinvitro.Thephagocytosisofperitonealmacrophagesethod;ObservedtheconcentratuonofNO-2/NO-3intheperitoneallavagefluid.ResultsDexamethasoneandcyclophosphamideinducedtheapoptosisofperitonealmacrophageandconitantdecreaseofphagocytosis(vscontrolgroup，P0.01)，theconcentratuono
3、fNO-2/NO-3intheperitoneallavagefluidsignificantlyincreasedafterdexamethasoneinjection;VitC(inhibitorofiNOSandinhibitorofreactiveoxygenspecies)preventedtheapoptosisofperitonealmacrophageandreducedtheconcentration 免疫抑制劑對小鼠腹腔巨噬細(xì)胞的影響論文.freelg/只，環(huán)磷酰胺2.0mg/只.freelethasoneandcyclphospham
4、ideonperitonealmacrophageofmice【Abstract】Objective)inducedbyinjectionofdexamethasoneandcyclophosphamideinmice.MethodsTheimmunefunctionbydeterminingthephagocyticfunctionandstructureofthemacrophagocytesoftheabdominalcavitiesofmiceinvitro.Thephagocytosisofperitonealmacrophagesethod;Ob
5、servedtheconcentratuonofNO-2/NO-3intheperitoneallavagefluid.ResultsDexamethasoneandcyclophosphamideinducedtheapoptosisofperitonealmacrophageandconitantdecreaseofphagocytosis(vscontrolgroup，P0.01)，theconcentratuonofNO-2/NO-3intheperitoneallavagefluidsignificantlyincreasedafterdexame
6、thasoneinjection;VitC(inhibitorofiNOSandinhibitorofreactiveoxygenspecies)preventedtheapoptosisofperitonealmacrophageandreducedtheconcentrationofNO-2/NO-3intheperitoneallavagefluidaftertheeffectofdexamethasone.ConclusionTheseevidencessupportthatNOandactiveoxygenspeciesmaybeinvolvedi
7、ntheapoptosisprocessofperitonealperitonealmacrophageinducedbydexamethasoneinmice.【Keyethasonecyclophosphamidemacrophage地塞米松和環(huán)磷酰胺在臨床腫瘤的治療中是常用的藥物，在殺傷腫瘤細(xì)胞的同時(shí)又會引起免疫系統(tǒng)損傷，而影響治療效果。巨噬細(xì)胞(macrophage，M)是機(jī)體免疫體系中重要的免疫效應(yīng)細(xì)胞，具有非特異性直接清除各種異物，殺傷病原體和腫瘤細(xì)胞的功能，并具有抗原提呈作用。本實(shí)驗(yàn)選用小鼠腹腔巨噬細(xì)胞進(jìn)一步探討在整體條件下地塞米松和環(huán)磷酰胺對
8、免疫功能影響的機(jī)制。1材料和方法1.1實(shí)驗(yàn)分組及動物
