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1、萬方數(shù)據(jù)ContentsABSTRACT??????????????????????????????????????????????IChapter1Review．．．，．．．．．．．?．?．．．，，．．．．?．．．．．．?．．．??．．?．．?．．??．??．．．．．．．．．．．．．．??．．．，．．?．．．．。．。．。．．．．?．．．．．．．。。11ResearchprogressofClavibactermichiganensissubsp．Nebraskense．．．．．．．．．．．??．?．I1．1CIavibactermichiganensissubsp．Nebraske
2、nse????????????????．．11．2Epidemiology?．．???．．．?．．．??．．．．?．．．．??．??．．．．?．???．．??．??．?．．?．．．?．．．．??11．3ResearchprogressofdetectionforClavibactermichiganensissubsp．Nebraskerise?????．．．?????．?????．????．??．??．????．??．??．??????．22ResearchprogressofPantoeastewartiisubsp．stewartii????．??．????．?．???22．1
3、Pantoeastewartiisubsp．stewartii????????????????????????．．22．2Epidemiology?．．．．．?．．．．．?．．．．?．．?．?．．???．．．．??．?．??．．．?．???．????．?．???32．3ResearchprogressofdetectionforPantoeastewartiisubsp．stewartii???．33Thecolloidalgoldimmunochromatographictechnology????????．??．????．43．1Characteristicandpreparat
4、ionofthecolloidalgold??????????????．43．2Preparationandapplicationofimmune-colloidalgold??．?．．?．????．．．??．53．3Compositionofimmunochromatographicstrip，．?．??。?．。????．?．．??．．??．63．4Principleandapplicationofthecolloidalgoldimmunochromatographictechnology??????．????．??．??．?????????????．??．????．??．???
5、?．64andsignificanceofstudy???????????????????????????．7Referances．．．．．．?．．．?．．．．??．??．．．?．．．．?．．????．．．．?．????．????．??．．??????．??．????．7Chapter2Thepreparationofthecolloidalgoldandimmune-colloidalgoId???121Purposeandsignificanceofstudy．?．??．?．??．??．??．????．．．??．??．?．．．?．．-．?．．122Materialsandmeth
6、ods??????????????????????????????????．．122．1Materials??????????????????????????????????????．．122．2Methods???????????????????????????????????????．13Resultsandanalysis．．．．．．．?．．．．．．．．．．．．．．．?．．．．．．?．．，??．．．．．．．．．．．．．．．．．．．．．?．．．?．．．?．．．．?．．．?．．．．．．．．．．．．．163．1Identificationofcolloidalgold????????
7、??????????????????163．2Determinationofconcentrationofantibodies．．．．．．．．．．?．．．?．．．．?．．．?．．．．?．．．．．．?．．．．163．3DeterminationofClavibactermichiganensissubsp．Nebraskenseimmune—colloidalgoldoptimizationpH?????????????????????????．???．16
