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《轉(zhuǎn)化生長因子β1胰島素樣生長因子1與髓核細(xì)胞誘導(dǎo)脂肪間充質(zhì)干細(xì)胞向髓核樣軟骨細(xì)胞分化的比較》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、萬方數(shù)據(jù)中國組織工程研究與臨床康復(fù)多爭似謄劈23婀2010—06—04出版JoumalofClinicalRehabilitativeTissueEngineeringResearchJune4J2010V01．14,No．23轉(zhuǎn)化生長因子131／胰島素樣生長因子1與髓核細(xì)胞誘導(dǎo)脂肪間充質(zhì)干細(xì)胞向髓核樣軟骨細(xì)胞分化的比較術(shù)★劉連江他，李放2，文天用1Transforminggrowthfactor-beta1／insulin-likegrowthfactor-1versusnucleuspulposuscellsininduci
2、ngthedifferentiationofadipose-derivedmesenchymalstemcellsintonucleuspulposus·likecellsUuLian-jian91上，LiFan92。Wennan·yon91Abstract8ACKGROUND：Previousstudieshaveaddressedadipose-derivedmesenchymalstemcellsfADSCs)culturewayformonolayerculture；inductionwaywasmainlycytoki
3、nemethod．a(chǎn)ndunderintervertebraldiscsmicro-environment．celIInteractionsalsodeservedfurtherstudyinitsthree-dimensionaIenvironment．inadditiontotheeffectsoncelIcytokines．OBJECTIVE：ToobservedifferenceslndifferentiationofADSCsintoNPCsundertwojnducedconditions：nucleuspulpos
4、uscells(NPCs)andtransforminggrowthfactor131(TGF-111)／insulin-likegrowthfactor(IGF-1)invitro．METHODS：RabbitADSCsmonolayercultureandNPCswereculturedrespectively。3generationsofADSCswerequalified：5xl0rcelIsweremadeintomicrospheresandplacedinTranswellcuttureplatesforcultu
5、reunderTGF．13111GF一1induction．CelIshapechangeswereobservedbefore，7，14daysafterinduction．Reversetranscription-polymerasechainreaction(RT·PCR)wasusedtodeterminelevelsoftypeIIcollagenandproteoglycan．RESULTSANDCONCLUSlON：At7and14days，nosignificantdifferencewasdetectedins
6、izeandshapeofADSCsmicrospheresinbothgroups．RT-PCRdetectionresultsdisplayedthattypeIIcollagenandproteoglycanmRNAexpressionwasdeterminedin7一dayTGF-BI／IGF-1groupandNPCs-inducedgroup．butexpressionwasstrongerintheTGF—DI／IGF-1group．Type¨collagenandproteoglycanmRNAexpressio
7、nwasobyiouslyincreasedin14一daVNPCs-inducedgroup。superiortoTGF-IBI／IGF-1group．Resultshavesuggestedthat／nvitroNPCsandTGF一131／IGF-1IeadtotheADSCs．whichhadpromotingeffectsonNPCsdifferentiation．TGF一131andIGF-1arenormaINPCs-secretedimportantfactors．Thepromotingeffectsonpro
8、liferationandd訐femntiationexist．withtheexceptionofcytokineinductioneftect，indifferentiationofADSCsintoNPCs．Ljuu，uF，WenTY．Transformi
