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1、動(dòng)物學(xué)雜志ChineseJournalofZoology 2008,43(5):104～109長(zhǎng)毛對(duì)蝦血細(xì)胞與血淋巴液的生化特性初步研究賈永紅 裘潔圓 周秋燕 陳吉?jiǎng)?浙江萬(wàn)里學(xué)院生物與環(huán)境學(xué)院 寧波 315100)摘要:長(zhǎng)毛對(duì)蝦(Fenneropenaeuspenicillatus)是我國(guó)東南沿海一帶一種主要的水產(chǎn)經(jīng)濟(jì)動(dòng)物。為了解其血淋巴液的基本特性,借助顯微觀察、生化實(shí)驗(yàn)等方法對(duì)長(zhǎng)毛對(duì)蝦的血細(xì)胞和血漿的細(xì)胞化學(xué)特征進(jìn)行了初步研究。結(jié)果表明,長(zhǎng)毛對(duì)蝦血細(xì)胞密度為(0143±0111)×107個(gè)Πml;可明顯分無(wú)顆粒細(xì)胞:長(zhǎng)徑(917±114)μm、短徑(518±018)μ
2、m,占細(xì)胞總數(shù)的1612%±118%;大顆粒細(xì)胞:長(zhǎng)徑(1113±113)μm、短徑(617±117)μm,占細(xì)胞總數(shù)的4616%±312%;小顆粒細(xì)胞:長(zhǎng)徑(711±014)μm、短徑(514±014)μm,占細(xì)胞總數(shù)的3517%±415%。在16～18℃下,60min的反應(yīng)時(shí)間內(nèi),血細(xì)胞對(duì)金黃色葡萄球菌(Staphylococcusaureus)菌體細(xì)胞懸液(106～107個(gè)Πml)的吞噬率為67%±5%。長(zhǎng)毛對(duì)蝦血漿蛋白濃度為11178mgΠml,對(duì)3%的雞血紅細(xì)胞懸液的溶血相對(duì)活性為216,對(duì)5%的雞血紅細(xì)胞懸液的凝集效價(jià)為15～18。血漿平板抑菌實(shí)驗(yàn)表明,長(zhǎng)毛對(duì)蝦
3、血漿對(duì)金黃色葡萄球菌有明顯的抗菌效果,抑菌圈大小為(10±1)mm,對(duì)大腸桿菌(Escherichiacoli)的抑菌圈大小為(6±2)mm。本工作為長(zhǎng)毛對(duì)蝦血漿中諸多活性多肽的后續(xù)研究奠定了基礎(chǔ)。關(guān)鍵詞:長(zhǎng)毛對(duì)蝦;血細(xì)胞;血漿中圖分類(lèi)號(hào):Q24,Q955 文獻(xiàn)標(biāo)識(shí)碼:A 文章編號(hào):025023263(2008)052104206BiochemicalPropertiesofHemocytesandHemolymphofFenneropenaeuspenicillatusJIAYong2Hong QIUJie2Yuan ZHOUQiu2Yan CHENJi2Gang(Col
4、legeofBiologicalandEnvironmentalSciences,ZhejiangWanliUniversity,Ningbo 315100,China)Abstract:Fenneropenaeuspenicillatusisoneofthemainaquaticcreatureswidelydisturbedinsouthern2eastcoastofNingbo,China.Tounderstandthecellularandchemicalcharacteristics,thehemocytesandhemolymphofF.penicillatus
5、wereisolatedandprimarilystudiedusingmicroscopeandchemicalanalysis.Theresultsshowedthatthedensityofhemocytewas(0143±0111)×107Πml.Atotalofthreetypesofhemocyteswereisolatedincludingagranulerhemocyte(AH):length917±114μm,width518±018μm,occupying1612%±118%ofthetotalhemocytes;large2granulehemocyt
6、e(LGH):length1113±113μm,width617±117μm,occupying4616%±312%ofthetotalhemocytes;small2granulehemocyte(SGH):length711±014μm,width514±014μm,occupying3517%±415%ofthetotalhemocytes.Undertheconditionof16-18℃and60minincubationtime,thephagocyticrateofhemocytesonStaphylococcusaureus(106-107Πml)was67
7、%±5%.Theproteincontentofhemolymphoftheshrimpwas11178mgΠml,therelativehemolysisactivityofthehemolymphto3%cockredcellswas216,andthecoagulantvalueofthehemolymphto5%cockredcellswas15-18.Thehemolymphshowedobviousantimicrobial基金項(xiàng)目 寧波市博士基金項(xiàng)目(No.2005A610026),寧波市自然科學(xué)基金
