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1、摘要本文以Eremophitabignoni毋Ol't2penth,JFMuell,的穗子、Eremophilamacdon—nelliiFMuetL的予葉和光禿愛(ài)沙木Eremophilaglabra(stickygreenleaf)的扦插藩豢芽莖段為舶棱俸送行縫織墻葵擅接孬生硬究。羞重磺究了壤揀霉生過(guò)程中的確動(dòng)培養(yǎng)、有效芽誘導(dǎo)、生根培養(yǎng)等各個(gè)階段的影響和制約因素,主幕包括基本壤纂鏊、生長(zhǎng)調(diào)節(jié)裁、糖、溪涯炭等,礁囊壅它弱在各令緒養(yǎng)狳羧酶最佳培莽基配方。并以Eremophilabignoniiflor
2、a(benth.●FMuell無(wú)菌蒴帶芽鍪段增熊培養(yǎng)居獲譬豹生蔽蕊進(jìn)J囂豫藹。主要磺究結(jié)票翅下:MS培辨基是種子和子葉啟動(dòng)培養(yǎng)中的效果都較好的基本培養(yǎng)基,種子萌發(fā)和予時(shí)分純最適寤動(dòng)培養(yǎng)蕊相閶均為MS*BAl.0mg/L+NAA0.Img/L。而B5培養(yǎng)基在扦插替帶芽莖段的啟動(dòng)培養(yǎng)中效果較好,最邋啟動(dòng)壤養(yǎng)基為B;+BA0.5mg/L+NAA0.Img/L。種子、子葉和扦插稿帶芽蒸段的啟動(dòng)率分別達(dá)到72.70%、7170%和79.70%。Eremophilabignoniiflora(benth.jFM
3、uelL無(wú)菌苗帶芽蒸段有效芽誘導(dǎo)的最佳培棼基配方為MS+BAl。0mg/L+NAA0.1mg/L。諺蝰葬基上奏效勞誘導(dǎo)率達(dá)73,0s%。60d時(shí)統(tǒng)計(jì),增殖倍數(shù)達(dá)5.5;EremophilamacdonnelliiFMuell子葉誘導(dǎo)有效芽熬最毽縫合戈麥芽糖+BAt.0mg/L+NAA0。lmg/L,該蹬養(yǎng)基上有效努誘導(dǎo)攀達(dá)7227%。60d時(shí)統(tǒng)計(jì),增殖倍數(shù)達(dá)5.0;光禿愛(ài)沙木扦插苗帶辯莖段有效芽誘導(dǎo)豹最佳潦養(yǎng)蓮醚方為KT0。5mg/L+BA0.5mgtL+IBA0。05mg/L。該培養(yǎng)鏊上的誘導(dǎo)舉達(dá)
4、67.23%。60d時(shí)統(tǒng)計(jì),增魑倍數(shù)達(dá)4.3。Eremophilabignoni(露口r4penth.jEMuelL生檄培養(yǎng)酌最饒配方為:1/2MS+NAAO.5mg/L+IBAO.05mg/L+1%活性炭,21d時(shí)統(tǒng)計(jì)生根率為81.6%,緞培茁完整植株的平均裉數(shù)為3.9,平均糧長(zhǎng)為3.1cm。Eremophilabignoniiflora(benth,)FMuelL最毽煉螢基質(zhì)為珍球裝:蛭石:溺沙=3:2:1,成活率達(dá)到80%以上。奩試驗(yàn)過(guò)程中,發(fā)瑗了予葉意接再生勞這孝孛本本檀物舔毒黲檀撩囂生方式
5、。關(guān)鍵逶:鬣沙本縫綴壤莽{耋揀菇生StudiesonTissueCultureandPlantletRegenerationofEremophilaspp.ZhangJiankang(ForestryCulture)DerectedbyProf.ShiDaxin91&AssociateProf.LuoJianxun2(1.CollegeofForestryandHorticulture,SichuanAgriculturalUniversity,Ya’an,Siehuan,China,6250142
6、.TheResearchlnstuteofForestry,SichuanAcademyofForestry,Chengdu,Siehnan,China,610081)AbstractThetissuecultureandplantletregenerationfromseedofEremophilabignoni夠·ora陽(yáng)enth.JFMuelI.,cotyledonofEremophilamacdonnelliiEMuellandsproutexplantswhichwerefromcutti
7、ngplantofEremophitaglabrafR3t)Ostenf(stickygreenLeaf)werestudied.Theinfluencefactorsofplantletregenerationwerestudiedduringtheinitiaiculture,budinductionandrootingculture。Throughanalyzingthebasalmedittm,phyto—hormone,saccharideandactivatedcharcoal,theo
8、ptimummediu—mwasconducted.Thetransplantationtrialofthetest—tubeplantletwhichderivedfromthebacteria—freeseedlingofEremophilabignoni孵orapenth.JEMuellwasmadeatlast.Themainresultsofthispaperwereconductedasfollows:MSW'aSthebestbasalmediumins