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1、陜西醫(yī)學雜志2014年8月第43卷第8期947草蓯蓉多糖提取物誘導人喉癌Hep2細胞凋亡的實驗研究延安大學附屬醫(yī)院耳鼻喉科二病區(qū)(延安716000)張軍張耀明△王正輝▲汪立摘要目的：探討草蓯蓉多糖提取物(BRP)對人喉癌Hep2細胞的抗瘤作用。方法：采用高通量色譜儀分析多糖提取物成分，流式細胞儀分析細胞周期及凋亡蛋白，westernblot檢測細胞凋亡相關(guān)蛋白變化。結(jié)果：高通量色譜分析儀發(fā)現(xiàn)BRP成分單一，BRP對細胞的抑制率呈時間和濃度依賴性。細胞周期分析發(fā)現(xiàn)BRP使細胞滯留于G。／O期。與對照組相比，不同濃度的BRP(100
2、～400g／m1)明顯誘導細胞的凋亡。Westernblot結(jié)果發(fā)現(xiàn)BRP作用后，pro—caspase一3，pro—caspase一8和pro—caspase一9蛋白分裂增加，同時死亡受體DR5和Bax表達增加，而Bcl一2表達減少。結(jié)論：研究發(fā)現(xiàn)BRP主要通過使Hep2細胞周期變化和凋亡來抑制細胞的生長，其途徑主要包括線粒體內(nèi)部途徑和死亡受體外部途徑來完成。主題詞喉腫瘤@Hep2細胞細胞凋亡【中圖分類號】R739．65【文獻標識碼】Adoi：10．3969／j．issn．1000—7377．2014．08．004Polvsa
3、ccharideOfboschniakiarossicainducesapoptosisonHep2cellsDepartmentofOtolaryngology，theAffiliatedHospitalofYah’anUniversity(Yan’an716000)ZhangJunZhangYaomingWangZhenghuietalABSTRACTObjective：Theaimofthisstudywastoexploretheanti-tumorpotentialofapolysaccharidei—solatedf
4、romBoschniakiarossica(BRP)inHep2humanlarynxsquamouscarcinomacells．Methods：Byanalyzingtheextractpolysaccharidesofhigh-throughputchromatography，cellcycleandapoptosiswereanalyzedbyflowcy—tometrytochangesinprotein，apoptosiswasdetectedbyWesternblotrelatedprotein．Results：H
5、ighperformancesize-exclusionchromatographyanalysisshowedthatBRPwasahomogeneouspolysaccharide．BRPsuppressedtheproliferationofHep2cellsinatime—anddose—dependentmanner．CellcycleanalysisrevealedthatexposuretOBRP(200Fg／m1)causedaG0／G1cellcyclearrestinHep2cells．Moreover，tr
6、eatmentwithBRPat100—400~g／mlfor24hinducedasignificantapoptosisHep2cellscomparedtOuntreatedcontrolcells，asdeterminedbyflowcytometrywithannexin-V／propidiumiodidedoublestaining．Additionally，BRPtreatmentpromotedthecleavageofpro～easpase一3，pro—caspase一8，andpro—caspase一9，co
7、upledwithincreasedexpressionofdeathreceptorDR5andBaxandreducedexpressionofBcl一2．Conclusions：Takentether，ourdatademonstratethatBRPshowspotentantitumoractivityinhumanlarynxsquamouscarcinoma，largelythroughinductionofG0／G1cellcyclearrestandapoptosis．Activationofbothmitoc
8、hondria—-mediatedanddeathreceptor-mediatedapoptosispathwaysisinvolvedinthecytotox、．icityofBRP．KEYWORDSLaryngealneoplasms@Hep2ceilsA
