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1、電針調(diào)控大鼠胃酸分泌的機(jī)制論文.freelL,P0.01],胃液酸度明顯降低[(30±3)vs(41±1)mmolL-1,P0.05],胃液pH升高.胃粘膜Gas含量增多[(136±17)vs(73±4)ngL-1,P0.01],血漿Gas含量下降[(84±22)vs(108±30)ngg-1,P0.01],胃液Gas無(wú)變化;胃液及胃粘膜EGF含量顯著升高[(3.2±1.1)vs(1.8±0.4)μgL-1和(5.1±0.8)vs(2.4±0.4)μgg-1,P0.01],血漿EGF無(wú)變化.胃壁細(xì)胞泌酸小管不擴(kuò)張,小管泡充盈不足,且數(shù)量不減少,非經(jīng)非穴組及空白對(duì)照組各項(xiàng)指標(biāo)無(wú)
2、顯著變化.結(jié)論電針足三里穴抑制大鼠胃G細(xì)胞Gas釋放,降低血漿Gas,增加胃粘膜、胃液EGF含量;電針足三里可改變胃壁細(xì)胞超微結(jié)構(gòu),抑制胃壁細(xì)胞泌酸小管功能、降低胃酸分泌.KeyonesAbstract:AIMToinvestigatetheregulativemechanismofelectroacupunture(EA)ongastricacidsecretioninrats.METHODSSDrats(n=75)izedinto5groups:Controlgroup,non-pointgroup,Sanyinjiaogroup,zasanligroup,andunit
3、egroup.Allratsinconsciousnessulatedbyelectroacupunctureexceptcontrolgroup.eanmunoassayalgrouthfactor(EGF)inplasma,gastricjuiceandgastricmucosatis-sue.Cellularultra-microstructureofgastricmucosacoatissionelectronmicroscope.RESULTSAfterEA,iteofthefastinggastricjuicesignificantlyreduced(0.30±0
4、.11)vs(0.63±0.12)mL,P0.01,andtheacidityofthegastricjuicesignificantlydropped(30±3)vs(41±1)mmolL-1,P0.05,andthepHofgastricjuicerose.EAstimulationofzusanliledtoadropofplasmicGas(84±22)vs(108±30)ngL-1,P0.01,ariseofGasingastricmucouscoats(136±17)vs(73±4)ngg-1,P0.01,butlittlechangeinGasingastric
5、juice.Besides,thereeotherchangesob-served.EGFofbothgastricjuiceandgastricmucouscoatin-creased(3.2±1.1)vs(1.8±0.4)μgg-1,P0.01,and(5.1±0.8)vs(2.4±0.4)μgg-1,P0.01respectivelyicEGFdidn’tchange,ber.Theaboveindexesinnon-pointgroupthoseofcontrolgroup(P0.05).CONCLUSIONEAstimulationofzusanlicaninhib
6、itGasrelease,reduceGascontentsinplasmaandgastricjuice,enhancecontentsofEGFingastricmucosaandjuice,changeultra-microstructureofgastricglandcells,andinhibitthese-cretoryfunctionofacinotubulesinparitetalcell.Finaly,Thegastricacidsecretionisdecreased.0引言中醫(yī)認(rèn)為,經(jīng)絡(luò)內(nèi)屬臟腑、外絡(luò)肢節(jié),胃與脾互為表里,相互影響,針刺經(jīng)絡(luò)腧穴,可激發(fā)內(nèi)
7、源性生理性調(diào)節(jié),影響飲食的受納、腐熟與運(yùn)化.現(xiàn)代實(shí)驗(yàn)研究表明,針灸對(duì)胃酸分泌具有調(diào)節(jié)作用,可能與某些胃腸激素有關(guān)[1].但針刺如何調(diào)節(jié)胃腸激素的變化,是否調(diào)節(jié)臟腑細(xì)胞分子結(jié)構(gòu)及超微結(jié)構(gòu),進(jìn)而影響胃酸分泌,尚未見(jiàn)有關(guān)研究報(bào)告.我們?cè)谟^察針刺后胃酸分泌變化的同時(shí)研究了胃泌素、表皮生長(zhǎng)因子含量及受體表達(dá)情況[2],觀察到胃腺細(xì)胞超微結(jié)構(gòu)的變化.旨在探討電針足三里穴對(duì)大鼠胃酸分泌的調(diào)控機(jī)制.1材料和方法1.1材料SD健康大鼠75只(本校實(shí)驗(yàn)動(dòng)物中心提供),體質(zhì)量200~250g,12~14in.對(duì)照組每日按規(guī)定