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1、黃?？嚓P(guān)細(xì)菌抗菌活性菌株的篩選及其初步分類鑒定416中田抗生素雜志2011年6月第36卷第6期文章編號:1001—8689(2011)06—0416—05黃?？嚓P(guān)細(xì)菌抗菌活性菌株的篩選及其初步分類鑒定劉祝祥黃苛肖懷東(植物資源保護與利用湖南省高校重點實驗室,陳奇輝賀建武陳義光吉首大學(xué)生物資源與環(huán)境科學(xué)學(xué)院,吉首416000)摘要:目的對分離自硇洲島黃?？?Anthopleura口Drn刪lc口)樣品的126株細(xì)菌進(jìn)行抗菌活性篩選和初步分類鑒定.方法以8種微生物(3株革蘭陽性菌,3株革蘭陰性菌
2、和2株真菌1作為指示菌,采用瓊脂擴散法篩選抗菌活性菌株,采用基于l6SrRNA基因序列的系統(tǒng)發(fā)育分析和生物學(xué)特性研究對其中抗菌譜較廣且抗菌活性穩(wěn)定的菌株進(jìn)行初步分類鑒定.結(jié)果67株菌具有抗菌活性,陽性率為53.2%,抗菌譜較J'且活性較強的9株,占受試菌株總數(shù)的7.1%.經(jīng)過形態(tài)觀察,生理生化特征及基于16SrRNA基因序列的系統(tǒng)發(fā)育分析表明,9株具有較強抗菌活性的菌株分別屬于芽孢桿菌屬(Bacillus1,鹽芽孢桿菌屬(Halobacillus),短桿菌屬(Brachybacterium1,擬
3、諾卡菌屬(Nocardiopsis1,鹽單胞菌屬(Halomonas),假交替單胞菌屬(Pseudoalteromonas)~弧菌屬(~brio)菌株.結(jié)論分離自中國南海的黃海葵相關(guān)微生物抗菌活性菌株具有豐富的類群多樣性.關(guān)鍵詞:黃?？?細(xì)菌;抗菌活性篩選;系統(tǒng)發(fā)育分析;鑒定中圖分類號:R378文獻(xiàn)標(biāo)識碼:AScreeningandpreliminaryidentificationoftheantimierobialstrainsassociatedwithAnthopleuraxanthogr
4、ammicaLiuZhu-xiang,HuangKe,XiaoHuai—dong,ChenQi-hui,HeJian—WU,ChenYi?guang(KeyLaboratoryofPlantResourcesConservationandUtilizationofHunanProvince,CollegeofBiologicalResourceandEnvironmentScience,JishouUniversity,Jishou416000)Abstract0biectiveToinvest
5、igatetheScreeningandpreliminaryidentificationof126antimicrobialstrainsisolatedfromAnthopleuraxanthogrammicacollectedfromtheareaoftheNaozhouislandintheSouthChinaSea.MethodsTheantimicrobialactivityofthestrainsweretestedusingtheagardiffusionmethodemploy
6、ingeightindicatorstrainsincludingSarcinalutea,Staphylococcusaureus,Escherichiacoli,尸teusvulgar~,Bacillussubtilis,Candidaalbican,AsperillusnigerandEnterobacteraerogenes.Thepreliminaryidentificationofthestrainswereinvestigatedbyusingphylogeneticanalysi
7、sbasedon16SrRNAgenesequencecomparisons.ResultsTheresultsshowedthat67strainsoutofthe126strainsexhibitedantibacterialactivity.inwhich9strainsexhibitedstrongantibacterialactivity.Basedontheresultsofmorphologiccharacteristics.physiologicalandbiochemicalp
8、ropertiesandphylogeneticanalysisof16SrRNAgene,9strainsbelongedtothegeneraBacillus,Halobacillus,Brachybacterium,Nocardiopsislomonas.PseudoalteromonasandVibriorespectively.ConclusionTheresultspresentedhereshowedthatthereisabundantdiversityofpopulationo
