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1、NaOH預(yù)處理對玉米秸稈纖維結(jié)構(gòu)特性和酶解效率的影響預(yù)處理對玉米秸稈纖維結(jié)構(gòu)特性和酶解效率的影響趙晶1,張福蓉1,陳明2*,徐晨1,權(quán)春善1,范圣第1圓援大連工業(yè)大學(xué)生物工程學(xué)院,(員援大連民族大學(xué)生命科學(xué)學(xué)院,國家民委原教育部生物化工重點實驗室,遼寧大連員員遠(yuǎn)遠(yuǎn)園園;遼寧大連員員遠(yuǎn)園猿源)摘要:為研究不同溫度下NaOH預(yù)處理對玉米秸稈纖維結(jié)構(gòu)特性和酶解得率的影響,為確定纖維原料堿法預(yù)處理的適宜條件提80、105益下預(yù)處理,供理論依據(jù),采用稀NaOH溶液對玉米秸稈分別在60、測定了預(yù)處理前后纖維原料的化學(xué)組成和酶解得率,并X射線衍射儀采用掃描電鏡(SEM)、(XR
2、D)和傅里葉變換紅外光譜儀(IR)對預(yù)處理前后玉米秸稈的纖維結(jié)構(gòu)進(jìn)行了表征。結(jié)果表NaOH預(yù)處理能夠有效脫除玉米秸稈中木質(zhì)素,明:增加纖維素和半纖維素比例,提高纖維素結(jié)晶度,產(chǎn)生的潤漲作用導(dǎo)致纖維束60h的酶解得率達(dá)到71.4%;狀結(jié)構(gòu)疏松。NaOH80益預(yù)處理1h后,玉米秸稈中纖維素結(jié)晶度達(dá)到63.7%,堿處理溫度進(jìn)一步升高則會充分暴露纖維表面紋孔,同時使纖維素分子內(nèi)氫鍵重新形成,容易進(jìn)一步損失半纖維素,降低纖維素的潤漲程度,從而降低酶解效率。80益條件下堿處理能夠有效改善玉米秸稈纖維結(jié)構(gòu),提高其轉(zhuǎn)化利用效率。NaOH預(yù)處理;關(guān)鍵詞:玉米秸稈;化學(xué)組成;纖維結(jié)構(gòu)
3、;酶水解X712中圖分類號:A文獻(xiàn)標(biāo)志碼:1672-204307-1407-06文章編號:(2016)doi:10.11654/jaes.2016.07.025EffectofNaOHpretreatmentsoncellulosicstructureandenzymatichydrolysisofcornstover(1.CollegeofLifeScience,DalianNationalitiesUniversity,KeyLaboratoryofBiochemicalEngineeringofStateEthnicAffairsCommission-Min
4、istryofEducation,Dalian116600,China;2.SchoolofBiologicalEngineering,DalianPolytechnicUniversity,Dalian116034,China)Abstract:Cornstoverbiomassisanimportantsourceofbioenergy.However,ligninincornstoverprofoundlyreducesitsenzymaticdegrada原turecharacteristicsandenzymatichydrolysisyieldsofc
5、ornstoverwereinvestigated.Thecellulosicstructuresofrawandalkali-pretreatedcelluloseandhemicelluloseandthecrystallinityofcellulose,resultinginmoreporouscellulosicstructure.Afterpretreatmentswithdilutesisto71.4%after60h.Highpretreatmenttemperaturecausedexposureofpitsonfibersurfaceandref
6、ormationofhydrogenbondsincellu原ticsandfacilitatingtheconversiontosaccharidesfromcellulose.ZHAOJing1,ZHANGFu-rong1,CHENMing2*,XUChen1,QUANChun-shan1,FANSheng-di1tion.Heretheinfluencesofsodiumhydroxidepretreatmentsunderdifferenttemperatures(60益,80益and105益)oncellulosicstruc原cornstoverwer
7、echaracterizedusingScanningElectronMicroscopy,X-rayDiffractometer(SEM)(XRD)andFourierTransformIn原fraredSpectrometer.Resultsshowedthatalkalinepretreatmentsofcornstovereffectivelyremovedlignin,andincreasedtheratiosof(FTIR)NaOHsolutionunder80益for1h,thecrystallinityofcelluloseofcornstover
8、resid