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1、華中科技大學(xué)碩士學(xué)位論文結(jié)論：n-3-PUFAs能夠降低血清TG、輕微降低血糖，但會導(dǎo)致體重、腹腔脂肪增加及肝臟脂質(zhì)沉積，其機(jī)理與n-3-PUFAs不能抑制SREBP-1c及其下游脂肪合成相關(guān)酶FAS及ACC的表達(dá)有關(guān)。故臨床上2型糖尿病患者在應(yīng)用n-3-PUFAs時(shí)需慎重。格列喹酮對肝臟脂質(zhì)沉積有改善作用?！娟P(guān)鍵詞】n-3多不飽和脂肪酸；2型糖尿??；脂質(zhì)沉積；SREBP-1c；5華中科技大學(xué)碩士學(xué)位論文TheInfluenceofn-3PolyunsaturatedFattyAcidsonLipidomicsofLiverinType2DiabeticR
2、atsCandidate：LuMinSupervisor：Prof.WenXiuyingAbstractObjective：Toinvestigatetheeffectofn-3polyunsaturatedfattyacidsonglucoseandlipidmetabolismandlipidomicsofliverintype2diabeticrats.Tostudyitsmolecularmechanismpossibly.Methods：50Wistarratsweredividedrandomlyintonormalcontrolgroup10
3、andmodel40afteroneweeksadaptivefeeding.Buildingtype2diabetesratsmodelwithhigh-fatandhigh-sugardietaddinjectionofstreptozotocin,thecontrolgroupwasinjectedbuffer.The28successfulmodelratsweredividedrandomlyintomodelgroup8,n-3-PUFAsgroupandgliquidonegroup10each.n-3-PUFAsgroupweregiven
4、n-3-PUFAs13.5g/kg/d;gliquidonegroupweregivengliquidoneaqueoussolution135mg/kg/d;modelgroupandnormalgroupweregivendistilledwatercontour;continuedtreatmentfor12weeks.Inthebeginningbeforeadministration,6weeksand12weeksafteradministration,weobservedthebodyweight(BW),serumtriglyceride(
5、TG),totalcholesterol(TC),fastingbloodglucose(FBG).Aftertheendoftheexperiment,weobservedtheitemofintra-abdominalfat,liverwetweight,andcalculatetheindexofintra-abdominalfatandliverindex,andtheliverchangesinthestructure;DetectedTGandTCcontentoflivertissues.RT-PCRdetectionthelivertiss
6、ueofSREBP-1c(SREBP-1c),fattyacidsynthase(FAS)andacetylcoenzymeAcarboxylase(ACC)geneexpression,Western-blotdetectionoflivertissueofSREBP-1cproteinexpression.Results：(1)Thechangeofbodyweightandbodyfat:After6weeksofadministration,gliquidonegroup’sweightwerereducedsignificantly,compar
7、edwiththenormalgroup(P<0.01).After12weeksofadministration,modelandgliquidonegr6華中科技大學(xué)碩士學(xué)位論文oup’sweightwereloss,comparedwiththenormalgroup(P<0.01orP<0.05);n-3-PUFAsgroup’sweightweregain(P<0.05).n-3-PUFAsgroup’sweightwereincreasedsignificantly，comparedwiththemodelgroup(P<0.01).n-3-P
8、UFAsgroup’sbodyfatwereincreased,c
