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1、14-不同寄主來源的灰葡萄孢對(duì)番茄的致病力分化研究不同寄主來源的灰葡萄孢對(duì)番茄的致病力分化研究李喜玲高智謀*李艷梅曹中革陳偉汪世軍?安徽農(nóng)業(yè)大學(xué)植物保護(hù)學(xué)院合肥230036摘要：從安徽合肥、蚌埠、長(zhǎng)豐、和縣等市、縣的番茄、辣椒、草莓、葡萄等發(fā)病寄主上分離鑒定獲得18個(gè)灰葡萄孢Botrytiscinerea菌株，采用菌絲塊創(chuàng)傷接種法，分別測(cè)定了上述不同寄主來源的灰葡萄孢菌對(duì)番茄果實(shí)和葉片的致病力。結(jié)果表明，所有供試菌株接種番茄果實(shí)后均引起發(fā)病，但不同菌株所致病斑的平均直徑有顯著差異，顯示灰葡萄孢菌株間
2、對(duì)番茄果實(shí)的致病力存在明顯分化。按照在番茄果實(shí)上所致病斑的平均直徑大小可將供試菌株致病力劃分為較強(qiáng)、中等和較弱3種類型?？傮w來說，來自番茄的菌株對(duì)番茄果實(shí)的致病力較強(qiáng)，來自草莓、葡萄和辣椒的菌株對(duì)番茄果實(shí)的致病力較弱，但來自相同寄主的菌株間致病力也存在差異，菌株致病力差異與菌株地域來源無明顯相關(guān)。供試灰葡萄孢菌株接種番茄葉片后，除CF1外，均可引起番茄葉片發(fā)病，但不同菌株所致番茄葉片病斑的平均直徑也有顯著差異；供試菌株對(duì)番茄葉片的致病力差異與菌株的寄主和地域來源無顯著相關(guān)。關(guān)鍵詞：番茄灰霉病，病斑平
3、均直徑，致病類型，地域來源DifferentiationofpathogenicityofBotrytiscinereastrainsfromdifferenthoststotomatoLIXi-LingGAOZhi-Mou?LIYan-MeiCAOZhong-GeCHENWeiWANGShi-JunCollegeofPlantProtection,AnhuiAgriculturalUniversity,Hefei230036,ChinaAbstract:EighteenisolatesofBotr
4、ytiscinereawereobtainedfromthediseasedplanttissuecollectedinHefei,Bengbu,ChangfengandHexianinAnhuiprovince,bymeansoftissueisolatingmethod.ThepathogenicityoftheisolatesofB.cinereafromdifferenthoststothefruitsandleavesoftomatowereinvestigatedbyapplyingwo
5、undinoculationwithmycelialblocks.Theresultsshowedthatallofthetestedisolatescausedgreymouldontomatofruits,buttherewassignificantdifferenceintheaveragediametersofthelesionscausedbydifferentisolates,suggestingthattherewassignificantdifferentiationinpathog
6、enicityofB.cinereastrainstotomatofruitsamongisolates.Accordingtotheaveragediametersofthelesionsontomatofruits,thepathogenicityoftheallisolateswasclassifiedintothreecategories:strong,intermediateandweak.Ingeneral,theisolatesfromtomatoweremorestronglypat
7、hogenictotomatofruitsthantheisolatesfromstrawberry,grapeandcapsicum.However,therewasdifferenceinpathogenicityamongthedifferentisolatesfromthesamehost,andthepathogenicitydifferencewasnotobviouslyrelatedtothelocalitiesofisolates.Afterinoculatingoftomatol
8、eaves,allofthetestedisolatesexceptCF3causedgreymouldontomatoleaves,buttherewassignificantdifferenceintheaveragediametersofthelesionscausedbydifferentisolates;andthedifferenceinpathogenicitytotomatoleaveswasnotobviouslyrelatedtothehostan
