資源描述：
《化學(xué)缺氧以及外源性血管內(nèi)皮生長因子對臍靜脈內(nèi)皮細(xì)胞可溶性血管內(nèi)皮生長因子受體-mrna表達(dá)影響的研究論文》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、華中科技大學(xué)碩士學(xué)位論文用呈現(xiàn)劑量依賴關(guān)系；3.VEGF在拮抗CoCl2誘導(dǎo)臍靜脈內(nèi)皮細(xì)胞缺氧損傷時(shí)，細(xì)胞內(nèi)sFlt-1mRNA表達(dá)強(qiáng)度下降，其作用呈劑量依賴關(guān)系。關(guān)鍵詞：妊娠高血壓疾??；人臍靜脈內(nèi)皮細(xì)胞；可溶性血管內(nèi)皮生長因子受體-1；血管內(nèi)皮生長因子3華中科技大學(xué)碩士學(xué)位論文StudyontheeffectsofchemicalhypoxiainducedbycobaltchlorideandextraneousVEGFoncellinjury,proliferationandtheexpressionofsolub
2、levascularendothelialgrowthfactorreceptor-1mRNAofhumanumbilicalveinendothelialcells.AbstractPart1Chemicalhypoxiaeffectsofcobaltchlorideoncellinjury,proliferationandtheexpressionofsolublevascularendothelialgrowthfactorreceptor-1mRNAofhumanumbilicalveinendothelia
3、lcells.Objective:investigatethechemicalhypoxiaeffectofcobaltchlorideonthecellinjury,proliferation,andtheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1)ofhumanumbilicalveinendothelialcells.Methods:cellinjurywasevaluatedbycellviability(MT
4、Tassay),lacticdehydrogenase(LDH)releasedfromallgroupHUVECsintothemediumweremeasuredbyusingLDHkits,andtheexpressionofsFlt-1ofallgroupwasdetectedbyusingthequantitativereversetranscriptase-polymerasechainreaction(RT-PCR).Results:cobaltchloridecouldinjuryHUVECs,induc
5、edtheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1),andtherewasatimedependentmanner.Conclusion:HUVECswereinjuredinducedbyCOCl2,proliferativeabilitywaslessenedandtheexpressionofsFlt-1mRNAwasup-regulated,theexpressionofsFlt-1mRNAofHUVECs
6、mayplayanimportantroleinthedevelopmentofhypertensivedisordercomplicatingpregnancy.Keywords:Hypertensivedisorderscomplicatingpregnancy;Humanumbilicalveinendothelialcell;solublefms-likekinasreceptor-1;chemicalHypoxia;4華中科技大學(xué)碩士學(xué)位論文Part2Theinvestigationontheagonistic
7、effectofextraneousVEGFontheinjuredHumanumbilicalveinendothelialcellsinducedbyCoCl2Objective:toinvestigatetheeffectofextraneousVEGFontheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1)ofhumanumbilicalveinendothelialcellsinducedbychemicalh
8、ypoxiawithCoCl2Methods：Thecellshadbeenculturedunderserumstarvationconditionfor12hour,whichwererandomlydividedintothefollowinggroups:①normalcontrolgroup(cellswe
