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1、CharacteristicsofEscherichiacolibio?lmproduction,genetictyping,drugresistancepatternandgeneexpressionunderaminoglycosidepressureswww.themegallery.comEscherichiacoli大腸桿菌infection感染Aminoglycoside氨基糖苷類(lèi)antibiotic抗生素Pseudomonas假單胞菌aeruginosa聚合酶鏈反應(yīng)技術(shù)Crystalvioletstai
2、ningmethod結(jié)晶紫染色法phenotype表現(xiàn)型drugresistancepattern耐藥模式Antimicrobialagent抗菌藥物molecularbiologyreagent分子生物學(xué)試劑Brothmicrodilutionmethod肉湯稀釋法Susceptibilitytesting藥敏試驗(yàn)Genotype基因型Real-timeqRT-PCR實(shí)時(shí)定量熒光PCRStreptomycin鏈霉素amikacin丁胺卡那霉素Gentamycin慶大霉素apramycin安普霉素1.Newwords
3、,www.themegallery.comBackgroundSeveraltypesofinfectioncausedbyEscherichiacoli(e.g.,urethralcatheter,biliarytractprosthesis,biliarytractinfection,lithangiuria,urinarytractinfectionandmanyotherdiseases)areassociatedwithbio?lmformation,whichleadstoaninabilitytoera
4、dicatetheinfectionduetoitsintrinsicnaturetoresisthighlevelsofantibiotics(FontaineandSmith,2006;Melchioretal.,2006)..www.themegallery.comBackgroundInaddition,underextensiveandpersistentpressureofantibiotics,inordertosurvive,theE.coliformsbio?lmtoevadetheimmunecl
5、earanceandresultsinmultipleantibioticsresistance.Hoffmanetal.(2005)foundthatsubinhibitorylevelsoftheaminoglycosideantibioticscouldinduceE.coliandPseudomonasaeruginosabio?lmformation.Someotherstudiesalsorevealedthatbio?lmformationbyE.coliinvitrocorrelatedwiththe
6、virulencefactors(Navesetal.,2008;Rijavecetal.,2008).www.themegallery.comAbstractInthisstudy,qualitative(scanningelectronmicroscope)andsemi-quantitative(modi?edcrystalvioletstainingmethod)methodshadbeenusedtoevaluateEscherichiacolibio?lm-formingability.Brothmicr
7、odilutionmethodandenterobacterialrepetitiveintergenicconsensus-basedPCR(ERIC-PCR)wereperformedtostudyE.colidrugresistancepatternandgenetictyping.Basedontheresultsabove,westudiedthecorrelationbetweenbio?lm-formingabilityphenotype,drugresistancepatternandgenetict
8、ypinginE.coli.Real-timeqPCR(qRT-PCR)wasusedtorevealmRNAexpressionlevelofE.colibio?lmrelatedmultipleantibioticsresistancegenes(acrA,agn43,csgA,csgD,ompFandpgaA)underdifferent
