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《雞cacna1s 基因snps 及其與部分屠體性狀的關(guān)聯(lián)性研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、雞CACNA1S基因SNPs及其與部分屠體性狀的關(guān)聯(lián)性研究張軍（溫州科技職業(yè)學(xué)院，浙江溫州325006）摘要：研究發(fā)現(xiàn)L型鈣離子通道α1亞單位（CACNA1S）與動物肌肉的收縮和發(fā)育存在一定的關(guān)系，但還未見雞CACNA1S基因的研究。利用PCR-SSCP技術(shù)，對蕭山雞、仙居雞、靈昆雞、蕭山雞×仙居雞雜交F1代、愛拔益加雞CACNA1S基因5′-調(diào)控區(qū)（5′-UTR）和外顯子1（exon1）首次進行多態(tài)性檢測，結(jié)合屠體性狀做關(guān)聯(lián)分析。結(jié)果表明試驗群體CACNA1S基因5′-UTR和exon1存在2個單核苷酸多態(tài)性（SNPs）位點與屠體性狀關(guān)聯(lián)：SNPs716位點
2、JJ基因型的胸肌率極顯著（P<0.01）大于KK基因型。SNPs1711位點YY基因型的全凈膛率和胸肌率顯著大于（P<0.05）XY和XX基因型；YY和XY基因型的半凈膛率和胸肌率極顯著（P<0.01）大于XX基因型。初步推斷CACNA1S基因可能是影響雞部分屠體性狀（全凈膛率、半凈膛率、胸肌率、腿肌率）的主效基因或與主效基因連鎖，并且這些突變位點可能被用作分子遺傳標(biāo)記選擇雞的屠體性狀。關(guān)鍵詞：雞；CACNA1S基因；PCR-SSCP；屠體性狀；關(guān)聯(lián)分析StudyonAssociationofSingleNucleotidePolymorphismsofCAC
3、NA1SGenewithPartialCarcassTraitsinChickenZhangjun(Wenzhouvocationalcollegeofscience&technology,Wenzhou325006,China)Abstract:Calciumchannel,voltage-dependent,Ltype,alpha1Ssubunit(CACNA1S)wasrelatedtothecontractionandgrowingdevelopmentofmuscle,buttherewerenotthestudyofCACNA1Sgeneinchi
4、cken.Singlenucleotidepolymorphismof5′-UntranslatedRegions(5′-UTR)andExpressedregion1(exon1)ofCACNA1Sgeneof30Xiaoshanchickensand30Xianjuchickensand28Linkunchickensand30F1offspringofXiaoshanandXianjuchickensand24AAchickenswasanalyzedbyPCR-SSCP，anditsassociationanalysiswithcarcasstrait
5、swasconductedforthefirsttime.Inresults,itwasfoundthat2Singlenucleotidepolymorphisms(SNPs)of5′-UTRandexon1ofCACNA1Sgene，anditswereassociatedwithcarcasstraitsoftheexperimentgroup.InthelocusSNPs716,BreastmusclerateofgenotypeJJwasverysignificantlybetterthanthatofgenotypeKK（P<0.01）.Inthe
6、locusSNPs1711,EvisceratedweightrateandbreastmusclerateofgenotypeYYwassignificantlybetterthanthatofgenotypeXYandXX（P<0.05）,Semi-evisceratedweightrateandbreastmusclerateofgenotypeYYandXYwereverysignificantlybetterthanthatofgenotypeXX（P<0.01）.ItwasconcludedfromtheresultsthatCACNA1Sgene
7、maybethemajorgeneaffectingcarcasstraits(Evisceratedweightrate、Semi-evisceratedweightrate、breastmusclerate、legmusclerate)ofchickenoritlinkswiththemajorgene，andthemutationlocuscouldbeusedasmoleculargeneticmarkerstoselectthechickensforcarcasstraits．Keywords:chicken;CACNA1Sgene;PCR-SSCP
8、;carcasstraits;corr
