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1、授予單位代碼10089學(xué)號(hào)或申請(qǐng)?zhí)?6179中國圖書分類號(hào)R361HebeiMedicalUniversity碩士學(xué)位論文學(xué)術(shù)學(xué)位IL-1β對(duì)大鼠終板軟骨細(xì)胞增殖的影響及機(jī)制研究研究生：梁小微導(dǎo)師：任韞卓教授專業(yè)：病理學(xué)與病理生理學(xué)二級(jí)學(xué)院：基礎(chǔ)醫(yī)學(xué)院2018年3月目錄中文摘要···········································································································1英文摘要·································
2、··········································································3英文縮寫···········································································································6研究論文IL-1β對(duì)大鼠終板軟骨細(xì)胞增殖的影響及機(jī)制研究前言···································································
3、·······························7材料與方法······························································································8結(jié)果·········································································································17附圖·········································
4、································································20討論·········································································································25結(jié)論·········································································································28
5、參考文獻(xiàn)·····························································································29綜述細(xì)胞凋亡在椎間盤退變中的分子機(jī)制·············································33致謝···································································································45個(gè)人簡歷·············
6、······················································································46IL-1β對(duì)大鼠終板軟骨細(xì)胞增殖的影響及機(jī)制研究摘要目的：相間盤退變?yōu)槎喾N因素綜合影響所致，有報(bào)道表明，軟骨終板發(fā)生鈣化所引發(fā)椎間盤營養(yǎng)物質(zhì)供應(yīng)下降為椎間盤啟動(dòng)的關(guān)鍵性因素。本實(shí)驗(yàn)通過培養(yǎng)大鼠椎體終板軟骨細(xì)胞，檢測ADAMTS-5蛋白的表達(dá)水平與IL-1β是否具有相關(guān)性，在IL-1β炎性因子的干預(yù)下，通過檢測磷酸化p65蛋白,p65蛋白含量的變化，來探究IL-1β是否促進(jìn)ADAMTS-5的
7、表達(dá)通過NF-κB信號(hào)通路，從而促進(jìn)終板軟骨細(xì)胞退變，為預(yù)防和治療椎間盤退變提供新的方法。方法：取4-6周齡大鼠椎體的終板軟骨細(xì)胞進(jìn)行純化，通過原代及傳代培養(yǎng)。選擇生長較好的3代細(xì)胞進(jìn)行實(shí)驗(yàn)，通過觀察細(xì)胞的形態(tài)，來描繪細(xì)胞的生長曲線。1.采用MTT法研究不同濃度的IL-1β對(duì)大鼠終板軟骨細(xì)胞增殖的影響。通過在紫外分光光度計(jì)上測定各孔光吸收值，以時(shí)間為橫坐標(biāo)，吸光值為縱坐標(biāo)，觀察細(xì)胞增殖變化。2.研究不同濃度的IL-1β（5ng/ml，10ng/ml、20ng/ml、40ng/ml）對(duì)大鼠終板軟骨細(xì)胞ADAMTS-5蛋白表達(dá)的影響。3.培養(yǎng)皿中加入10ng/mL的
8、IL-1β，觀察24、4
