紅酵母高細胞密度酵母多糖提取工藝論文

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1、畢業(yè)論文紅酵母高細胞密度酵母多糖提取工藝39畢業(yè)論文摘要本實驗采用的是CICC編號31229的紅酵母，通過對紅酵母的實驗，了解紅酵母的特性及生長特點，進而改善紅酵母的培養(yǎng)條件及培養(yǎng)基來擴大紅酵母的生長濃度。研究從紅酵母中提取酵母多糖的提取工藝，確定酵母多糖提取方法。采用搖瓶培養(yǎng)的方法，通過碳源、氮源及主要營養(yǎng)與環(huán)境條件等對紅酵母產菌量的影響試驗，以及葡萄糖、蛋白胨、酵母膏三因素三水平的正交試驗，獲得了紅酵母的最佳生長條件：葡萄糖2％，蛋白胨1．0％，酵母膏0,5％，磷酸二氫鉀0．15％，硫酸鎂0．01％，氯化鈉1．0％，初始pH5．0，接種量10％，500mL三角瓶中培養(yǎng)基裝量為

2、80mL，發(fā)酵溫度為28℃，搖床200r／min。在此優(yōu)化的培養(yǎng)條件下，海洋紅酵母的產菌量可達9．7億個／mL，比優(yōu)化前發(fā)酵培養(yǎng)基產菌量（5．6億個／mL）提高了73．2％，比單因素試驗結果（8．1億個／mL）提高了19．8％。研究從酵母中提取胞壁多糖的提取工藝。提取工藝路線為：酵母溶解—凍融一超聲波破碎一堿溶一中和一沉淀一洗滌—烘干。通過正交試驗對酵母破壁和堿溶條件進行優(yōu)化。初步尋求提取工藝條件，多糖得率為19．4％，用苯酚一硫酸法測定多糖的含量為51．9％。關鍵詞:紅酵母高細胞密度培養(yǎng)條件優(yōu)化酵母多糖提取工藝39畢業(yè)論文AbstractTheexperimentwasthro

3、ughthestudyoftheRhodotorulaglutinis，tounderstandthecharacteristicsandgrowthcharacteristicsofRhodotorulaglutinis,Therebyimprovingconditionsforthecultivationofredyeastandthemediumtoexpandthegrowthofredyeastconcentration.Aflaskoftrainingmethods，TheuseofRhodotorulaglutinis，Throughthecarbonsource,

4、thenitrogensourceandthemainnutritionandtheenvironmentalconditionandsoonproducethefungusquantitytotheRhodotorulaglutinisbenthicatheinfluenceexperiment,aswellasthesucrose,theproteinpeptone,theyeastpastethreefactorsthreelevelsorthogonalexperiments,wehaveobtainedtheRhodotorula＇sbestfermentationco

5、ndition：sucrose1.5%,proteinpeptone1.0%,yeastpaste0.5%,potassiumdihydrogenphosphate0.15%,magnesiumsulfate0.01%,sodiumchloride1.0%,initialpH5.0,inoculatingquantity8%,80mLmediuminthe500mLtrianglebottle,temperature28℃,200revolutions/minute.Underthisoptimizedcondition,thenumberofRhodotorulahasreac

6、hed970million/mL,whichenhanced73.2%totheinitialconditi（560million/mL）andimproved19.8%totheresultofthetestofthesinglefactor（810million/mL）.Thisexperimentisstudyingonthecraftofpolysaccharidesextractionfromrhodotorulcell-wal1．Thecraftroutedrawn：yeastdissolve—freezethawing—uhrasoinccrush—alkaliab

7、straction—neutralize—deposit—solventwash—dry．Seekthebestcraftcondition，aswellasoptimizeconditionsofcrushwallandalkalidissolvingbyorthogonalexperiment．yieldofpolysaccharideis19.4％．Determinationwithphenol—sulphuricaicdlaw．contentofpolysaccharid