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1、體內構建骨軟骨復合體修復骨軟骨缺損的實驗研究【摘要】探討軟骨組織工程方法修復骨軟骨缺損理想的種子細胞和支架材料。［方法］體外誘導骨髓基質干細胞向軟骨細胞定向分化，分別與PLGA支架復合培養(yǎng)，并模仿馬賽克骨軟骨移植術在犬關節(jié)骨軟骨缺損深層置入MSC—支架復合體，淺層置入誘導培養(yǎng)后的MSC_支架復合體，緊密壓配，體內構建骨軟骨復合體，觀察其修復的情況。［結果］16周實驗組缺損區(qū)完全由透明軟骨修復，與周圍正常軟骨完全融合，組織觀察顯示以軟骨細胞修復為主，軟骨下骨形成，潮線基本恢復；陰性對照組缺損區(qū)主要由纖維組織修復，部分由透明軟骨修復，組織觀察
2、顯示以纖維細胞修復為主，混有部分軟骨細胞；空白對照組完全由纖維組織修復。［結論］MSCs經向軟骨細胞定向分化后復合PLGA支架材料，通過緊密壓配方式與MSCs-PLGA支架在體內構建骨軟骨復合體，可以有效修復骨軟骨缺損?！娟P鍵詞】骨髓基質干細胞馬賽克移植術骨軟骨復合體骨軟骨缺損Abstract:［Objective］Toevaluatetheeffectoftreatingtheosteochondraldefectswithimplantedcellscaffoldcomposites,culturedMSCsasseedcellsan
3、dPLGAasscaffolds,andtoacquiredesirableseedcellsandscaffoldmaterials.[Method]BMSCswereinducedtodifferenciatiatedintochondrocytes,coculturedwithPLGAscaffoldrespectivelyinvitro,thenimplantedintoosteochondraldefectsoncaninemodelsbyusingtechniquesofmosaicplasty,inducedBMSCPLGA
4、scaffoldcompositesinthetopofthedefectandBMSCPLGAscaffoldcompositesinthebottomofthedefect,osteochondralcompositeswereconstructedinvivo,andrepairwasobservedwithnakedeyesandhistology.[Result]Atl6weeksaftertransplantationthedefectsofexpEirmentalgroupwerecoveredwithsemitranspa
5、rentsmoothwhitetissueandthemarginsbetweentherepairtissueandthesurroundingcartilagewerenotrecognized.Histologically,mostoftherepairtissuewasconsistedwithchondrocytes,maintainedthEIrthicknesstothefulldepthoftheoriginaldefects.Thesubchondralbonewaswellremodeled.Thetidemarkwa
6、sobserved.Thedefectsofpositivecontrolgroupwerecoveredwithrepairtissues,andpartialwereconformedwithoriginalcartilage.Therepairtissuewaspartlyfilledwithchondrocytes.However,thedefectsofnegativecontrolgroupwererepairedwithsoftfibroustissueswithoutluster,andanobviousboundaryb
7、etweenreparativeandoriginalcartilagewasseen.[Conclusion]MSCsPLGAscaffoldcomposites,constructedintoosteochondralcompositesbysuppressingcloselyinvivo,aretheidealmaterialsforrepairingcartilagedefects.Keywords:bonemesenchymalstemcells(MSCs)；mosaicplasty；osteochondralcomposite
8、s；osteochondraldefect關節(jié)軟骨損傷是骨科臨床的常見疾病，以往臨床使用的治療方法均難以迗到理想的要求。組織工程技術的迅速發(fā)展，為這一難題的解決提供了前所未有的機遇。本實驗研究
