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1、華中農業(yè)大學2014屆碩士研究生學位論文miscanthus>waterpeanut>metasequoiachips.Inanyofthecellulaseloadings(7—200FPU/gcellulose),cellulosefrommetasequoiachips、^,itllthehighestcrystallinityindexhadtheminimumreducingsugaryield(20—60∥1009),meanwhile,thesugarcanebagassecellulose謝tllthelowestcrys
2、tallinityindexhadthemaximumreducingsugaryield(>85.72g/1009).Thehigherthecrystallinityindexwas,thehigherdegreeofcrystallizationwouldbe,andthemoredifficultthecombinationofenzymeandcelluloseWas,leadingtoalowercelluloseconversionandalowerreducingsugaryield.Anobviousnegativeco
3、rrelationbetweenCrIandhydrolysisyieldwasobtained、析tllthelinearcorrelationcoefficientRashighasO.9828,atsufficientlyhighenzymeloadingsof140and200FPU/gcellulosewheretheenzymeavailabilitywasnotalimitingfactorofthehydrolysisreaction.(3)Itwasfoundthatthecrystallinityindex(CrI)o
4、fplantcelluloseplayedagreaterrolethandegreeofpolymerization(DP)andspecificsurfacearea(SSA)did,inenzymehydrolysisbythemultiplelinearregressioncorrelationequationfittingbetweenthereducingsugaryield(Ⅵandstructuralcharacteristics(CrI,DP,SSA),meanwhile,theDPandSSAonlypresented
5、aminordegreeofpositivecorrelation、析tllthereducingsugaryield,thushadaminorinfluenceonthehydrolysisofplantcelluloses.TheinfluenceofstructuralcharacteristicsonenzymatichydrolysisWasintheorderofCrl>DP>SSA.Thispointstothepotentialadvantagesinpracticalapplicationofutilizingwate
6、rhyacinthasfeedstockforbio—ethanolproductionasithasrelativelylowCrIandrelativelyhighSSA.Whenwaterhyacinthisappliedtoenzymatichydrolysis,itCannotonlyreducethecostofenzymereagentaswellasthecostofbio-ethanolproduction,butalsocontroltheaquaticenvironmentalproblems.(4)Differen
7、tintensityofphosphoricacidandmicrowavepretreatmentswereemployedtowaterhyacinthcelluloseseparatedbyethanol—nitratemethodinordertochangeitsstructttralfeature.TheSSAofthepretreatedmaterialsdecreasedsignificantly,andthereducingsugaryieldsofthesematerialswerealsolowerthanthose
8、ofuntreatedcellulose.Therefore,waterhyacinthcelluloseseparatedbyethanol—nitratemethoditselfwasal