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《小鼠未成熟卵母細胞染色質(zhì)構(gòu)型及其和體外成熟能力和發(fā)育能力關(guān)系的研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、StudiesonchromatinconfigurationofunmaturedmouseoozeanditsrelationshipwiththemeiosisanddevelopmentcompetenceAbstractTherearemoreevidenceshowthatvafiouscstageofantra]follicularoncytebeforematurationcultureinvitro．firstreflectedonthechromminconfigurationofthenucleus，maya
2、ccountfor慨observedflawofinvitromaturationsyslelm。Inthisexperiment．westudiedthechromatinconfigurationchangesduringoogancsisanditsrelationshipwithovulation、meioticcompetence、fertilizationandpreimphntafiondevelopment．n七resultsa托越follows：1)Therearefourtypeschromatinconfig
3、ur蝴uninantralfolliculmooc難isolatedadultmouse．neyarenamedSN、NSN、pSN、pNSN．；SN、NSNtypeoocymismo∞frqncncythanpSN、pNSNtypeoncyte；2)TheappearanceofSN--ooeyteisfirstobunvedin17--day-oldmouse,thera把ofSN-oocyteissignificantly．ma℃asedwhenthellnouseagereachedlgand19days；3)Oocyte
4、indiameterbetween20Innand60)uuisfoundtobeNSN--tmcyte，butwhenOOCyteindiameterreached701am,SN-oocyteisfirstfoundandticrateofSN-oocyteisincreasedaStheOOCytcdiameterreached70---80¨m；11嘲℃aremorepSN和pNSN-oocytewhentheoogyeindiameterissmallerthanthatof70lain；4、PMSGinjectiona
5、ppearedtoinduceanincreaseofSN--ooeyleindiameter70-gOpmandaodecreaseofNSN-oocyteindiameter60^101,tm；ButhCGinjectionindHOgⅫdecreaseofSN—oocyteandbacktOasituationpriortoPMSGinjecfion．5、BothSN．．oocyteandNSN-oocyteisolatedfromarrmdfollicular鋤undergoGVBDandprogresstouptomet
6、aphaseILButthemeioticc嘰p咖nceofSN-oncyteishigherthanthatofNSN·oocyte；61Thefertilizationrateisnotsigdificant／ydifferentinbothgroupsofm謝刪oncytederivedfromSN—andNSN-oocyte；Bmthe2-celldevelopmentrateofSN-occyteishigherthanthatofNSN-oocyte；71TherateofSN—oocytcinantra／follic
7、ularisnotsignificantlydifferentbetwom6．8weeksoldand30-32woeksoldmouse；8)Withageing,therateofSN-oocytcisolatedfromanlralfollicularisincreased．Keyword：chromatinconfiguration，GV-stageooeyte，moose．2．Postgradua恤：YiYalingAdvisor：．Prof．HaoYanhong一旦蘭堡塵墨圭塑塑墨塑塞銎魚堊塑皇絲墨蘭墮苧些壟：莖!墮查
8、叁墨墮堡塞壁主笙苧前言胚胎工程操作如胚胎體外生產(chǎn)、胚胎克隆等研究所需要的大量成熟卵母細胞主要是由卵母細胞體外成熟(1vM)提供的．盡管許多種動物的IVM系統(tǒng)取得了根大的進展，目前的ⅣM系統(tǒng)還并不十分完善，這在豬和馬尤為突出．側(cè)如母馬卵母細胞體外成熟
