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《自體成肌細(xì)胞對創(chuàng)傷性聲門關(guān)閉不全恢復(fù)影響的實驗分析》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、-關(guān)鍵詞：成肌細(xì)胞聲門關(guān)閉不全甲杓肌聲帶3---Theeffectsofautologousskeletal
myoblaststransplantationonhurtedvocalcordofparalysisAbstractObjective:Thegoalofthepresentexperimentalstudyistoinvestigateeffectonadultrabbitautologousskeletalmyoblastsinjectionintothethyroarytenoid(TA)muscleafterrecurrentlaryngealnerve(RLN)injur
2、yandscarvocalcordMethods:Adultbigwhiterabbit，scleidomastoidalmasclemyoblastswascultivated、segregatedandexpand.Theleftsidebigwhitetherabbitneckwasmakeahorizontalnick,longabout4cm，Onebyoneinordercutopenaskin,subcutaneoustissue,separateaheadoftheneckmusclegroup,andfindoutrecurrentlaryngealnerveinthedit
3、chofwindpipeoesophagusatthethyroidglandbottom，atthe2-4trachealringlevelcutoffrecurrentlaryngealnerve1m,thenearheartrecurrentlaryngealnervewassewinsubcutaneouslyandsewupnickaftertakingoutleftsidecleidomastoidalmascle(providecleidomastoidalmasclemyoblasts).itwastoexposeglottisunderrisingepiglottiswith
4、thelaryngoscopeandrowascarattheleftsidevocalcordsurfacewihhthehookedneedle,thedeptharrivedvocalcordmuscle.Theestablishedanimalmodelaboutrecurrentlaryngealnerve(RLN)injuryandscarvocalcord.20bigwhiterabbitrandomweredividedinto2groups,each10,20bigwhitetherabbitallwerecutoffrecurrentlaryngealnerve(RLN)a
5、ndhurtedtheleftsidevocalcord.Agroupwasinjection4---skeletalmyoblastsintheleftthyroarytenoid(injectionskeletalmyoblastsgroup),therightsidewasnormalcompare;BgroupwasinjectionSodiumChlorideintheleftthyroarytenoidintheleftthyroarytenoid(injectionSodiumChloridegroup),therightsidewasnormalcompare.Toexamin
6、eAgroupandBgroupthyroarytenoidmusclefiberdiameterandvocalcordvolumeafter12weeks.Results:Didn'tshowdifference(p>0.05)fromitselfdoublemusclefiberdiameterandvocalcordvolumeofinjectionautologousskeletalmyoblastsanddidshowdifference(p＜0.05)fromdoublemusclefiberdiameterandvocalcordvolumeofinjectionSodiumC
7、hlorideafter12weeks.Conclusion：injectionautologousskeletalmyoblastsinthyroarytenoidmusclemademyoblastsbecomeobviousincreaseandlivearoundthyroarytenoidmusclefiberafter12weeksfromimmunohistochemistryund
