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1、專業(yè)資料分享連接互相匹配的粘性末端，產(chǎn)生新的酶切位點(diǎn)通常連接互相匹配的粘性末端可以產(chǎn)生新的酶切位點(diǎn)。以下組合是通過(guò)計(jì)算機(jī)程序計(jì)算出來(lái)的，盡管我們希望能盡量保證其準(zhǔn)確性，但有些未經(jīng)實(shí)驗(yàn)證實(shí)，因此不能保證100%的可行性。如果有同裂酶存在，則只列出其中的一個(gè)酶。本表中列出的內(nèi)切酶均為已商品化的內(nèi)切酶。識(shí)別序列不夠嚴(yán)謹(jǐn)?shù)膬?nèi)切酶（例如，識(shí)別多個(gè)序列的酶）只在括號(hào)內(nèi)標(biāo)出相關(guān)的序列，但注意這些酶不只切割該序列。"-"表示連接產(chǎn)物不能被再切割。酶連接到可進(jìn)行切割的酶Acc65I(G/GTACC)BanI(G/GTACC)Ac
2、c65I,BanI,KpnI,NlaIV,RsaI?BsiWI,BsrGIRsaIAccI(GT/CGAC)AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI--(GT/CGAC)ClaI,BstBI,TaqITaqIAciI(C/CGC)AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI--BsaHI(GR/CGCC),HinP1I,NarIAciIHpaIIHpaIIAclI(AA/CGTT)AccI(GT/CGAC),AciI,ClaI,BstBI,Hi
3、nP1I,HpaII,NarI,TaqI--AflIII(A/CGCGT)AscI,BssHIIBstUI,HhaI完美DOC格式整理專業(yè)資料分享(A/CATGT)BspHI,NcoI,PciINlaIII(A/CGCGT)MluIAflIII,BstUI,MluIAgeI(A/CCGGT)AvaI(C/CCGGG),XmaIHpaII,NciI,ScrFIBsaWI,BspEIBsaWI,HpaIIBsrFI(A/CCGGT),SgrAI(CA/CCGGTG)AgeI,BsaWI,BsrFI,HpaIINgo
4、MIVBsrFI,HpaIIApaI(GGGCC/C)BanII(GGGCC/C),Bsp1286I(GGGCC/C)ApaI,BanII,Bsp120I,Bsp1286I,HaeIII,NlaIV,Sau96IApaLI(G/TGCAC)SfcI(C/TGCAG)BsgIApoI(A/AATTY)EcoRIApoI,Tsp509I(G/AATTY)EcoRIApoI,EcoRI,Tsp509I(R/AATTY)MfeI,Tsp509ITsp509IAscI(GG/CGCGCC)AflIII(A/CGCGT),
5、MluIBstUI,HhaIBssHIIBssHII,BstUI,Cac8I,HhaIAseI(AT/TAAT)BfaI,Csp6I,NdeI--MseIMseIAsiSI(GCGAT/CGC)BsiEI(CGAT/CG)DpnII,PvuIPacIMseIPvuIDpnII,PvuI完美DOC格式整理專業(yè)資料分享AvaI(C/CCGGG)AgeI,BsaWI,BspEI,BsrFI(R/CCGGY),NgoMIV,SgrAI(CR/CCGGYG)HpaII,NciI,ScrFI(C/TCGAG)XhoIAva
6、I,TaqI,XhoI(C/TCGAG)SalITaqI(C/CCGGG)XmaIAvaI,BsaJI,HpaII,NciI,ScrFI,SmaIAvaII(G/GWCC)PpuMI(RG/GACCY)AvaII,NlaIV,Sau96IRsrIIAvaII,Sau96IPpuMI(RG/GTCCY)AvaII,BsmFI,NlaIV,Sau96IAvrII(C/CTAGG)NheI,SpeI,XbaIBfaIStyI(C/CTAGG)AvrII,BfaI,BsaJI,StyIBamHI(G/GATCC)Bcl
7、I,DpnIIAlwI,DpnIIBglII,BstYI(R/GATCY)AlwI,BstYI,DpnIIBstYI(G/GATCC)AlwI,BamHI,BstYI,DpnII,NlaIVBanI(G/GTACC)Acc65IAcc65I,BanI,KpnI,NlaIV,RsaI(G/GCGCC)KasIBanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV(G/GTACC)BsiWI,BsrGIRsaIBanII(GGGCC/C)ApaI,Bsp1286I(GGGCC/C)ApaI,Ba
8、nII,Bsp1286I,HaeIII,NlaIV,Sau96I(GAGCT/C)Bsp1286I(GAGCT/C),SacIAluI,BanII,BsiHKAI,Bsp1286I,SacI完美DOC格式整理專業(yè)資料分享BclI(T/GATCA)BamHI,BstYI(R/GATCY)AlwI,DpnIIBglII,MboIIDpnIIBfaI(C/TAG)AseI,Csp6I,Mse
