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《構(gòu)建nt-3交聯(lián)脫細(xì)胞脊髓支架緩釋系統(tǒng)及研究其體外實驗》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、目錄縮略語表1英文摘要2中文摘要6論文正文構(gòu)建NT-3交聯(lián)脫細(xì)胞脊髓支架緩釋系統(tǒng)及研究其體外實驗9第一章前言9第二章大鼠脫細(xì)胞脊髓支架制備方法的優(yōu)化．．112．1材料與方法112．2結(jié)果152．3討論．202．4小結(jié)．22第三章制備NT-3交聯(lián)脫細(xì)胞脊髓支架及其緩釋性研究233．1材料與方法233．2結(jié)果273．3討論．313．4小結(jié)．33第四章NT-3交聯(lián)脫細(xì)胞脊髓支架對大鼠骨髓間充質(zhì)干細(xì)胞粘附和增殖影響的研究．344．1材料與方法344．2結(jié)果364．3討論．394．4小結(jié)．39全文結(jié)論．40參考文獻．41文獻綜述組織支架修復(fù)脊髓損傷的研
2、究進展．．45參考文獻．49攻讀研究生期間發(fā)表的論文．．5l致謝．52萬方數(shù)據(jù)第三軍醫(yī)大學(xué)碩士學(xué)位論文縮略語表英文縮寫英文全稱中文全稱SCIspinalcordinjury脊髓損傷MSCSmesenchymalstemcells間充質(zhì)干細(xì)胞HEhematoxylinandeosin蘇木素和伊紅ECMextracellularmatrix外細(xì)胞基質(zhì)BMSCsbonemarrowmesenchymalstemcells骨髓間充質(zhì)干細(xì)胞PBSphosphatebufferedsaline磷酸鹽緩沖液SEMscanningelectronmicros
3、cope掃描電子顯微鏡EDTAethylenediaminetetraaceticacid乙二胺四乙酸FBSfetalbovineserum胎牛血清0Dopticaldensity光密度bFGFbasicfibroblastgrowthfactor堿性成纖維細(xì)胞生長因子DAPI4’，6．diamidino．2．phenylindole4’，6-二脒基-2-苯基吲哚AECMacellularextracellularmatrix脫細(xì)胞基質(zhì)EDC·HCl1．(3．Dimethylaminopropyl)碳二亞胺鹽酸鹽-3-ethylcarbodi
4、imidehydrochlorideNHSN．HydroxysuccinimideN．羥基琥珀酰亞胺DMEM／F12Dulbecco·SmodifiedEaglemedium：杜氏改良伊格爾培養(yǎng)基營NutrientMixtureF’-l2養(yǎng)F．12混合物NT-3neurotrophicfactor一3神經(jīng)營養(yǎng)因子．3mlmilliliter毫升ulmicrolitre微升rpmrotationperminute轉(zhuǎn)吩℃degreeCelsius攝氏度萬方數(shù)據(jù)第三軍醫(yī)大學(xué)碩士學(xué)位論文ConstructionofNT-3crosslinkedace
5、llularspinalcordscaffolddeliverysystemandexperimentalstudyofitscharacteristicinvitroAbstractBackground：Spinalcordinjury(SCI)isaseriouscentralnervoussystemtrauma．Inrecentyears，tissueengineeringprovidesanewwayforthetreatmentofSCI，composingofscaffold，seedcellandneurotrophicfac
6、torsadditional，contributingtotherecoveryofspinalcordfunction．Theprojecthasanimportantroleintissuerepairandthescaffoldprovideasuitablemicroenvironmentofmechanicalsupportforseededcelladhesion，growthandSOon．Currently,itisnaturaldegradationorsyntheticpolymerorcompositematerials
7、thatusedforspinalcordscaffold，suchas：hyaluronicacid，chitosan，PLGAsilkprotein．chitosancompositescaffoldmaterials．Thesematerialshavetheirownadvantages，buttheydon’thaveauniquethree—dimensionalnetworkstructureofthespinalcord，limitingintherepairofspinalcordreconstruction．Inprevi
8、ousstudies，wedecellularizedratspinalcordtoretaincollagen，fibronectin，lamininandoth
