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1、編號201006168刊用形式論著Notch-1對大鼠嗅球成鞘細胞神經(jīng)生長因子表達的影響卓飛,孫善全，余維華，劉輝，邱國平，楊美（400016重慶,重慶醫(yī)科大學人體解剖學教研室，神經(jīng)科學研究中心）[摘要]目的觀察Notch-1對嗅球成鞘細胞（olfactoryensheathingcells，OECs）神經(jīng)生長因子（nervegrowthfactor，NGF）表達的影響。方法原代培養(yǎng)大鼠OECs，用有活性的Notch質(zhì)粒（NotchICV），無活性的Notch質(zhì)粒（NotchVK）以及空質(zhì)粒分別轉染OECs，
2、將實驗對象分成活性質(zhì)粒組，非活性質(zhì)粒組和空白組，應用免疫熒光雙標、Westernblot及RT-PCR技術，檢測各組OECs中NGF及其mRNA的表達變化。結果轉染Notch-1后，OECs中NGF的表達明顯增高。免疫熒光雙標結果顯示，活性質(zhì)粒組OECs中NGF的表達較非活性質(zhì)粒組和空白組均明顯增高（P<0.05）；RT-PCR結果顯示，活性質(zhì)粒組（0.4961±0.0269）OECs中NGFmRNA的表達均高于非活性質(zhì)粒組（0.2445±0.0134）及空白組（0.2459±0.0160）（P<0.01）；
3、Westernblot結果顯示，活性質(zhì)粒組（0.8875±0.0166）OECs中NGF的表達均高于非活性質(zhì)粒組（0.6862±0.0592）和空白組（0.6821±0.0670）（P<0.01）。結論Notch-1轉染OECs后，其NGF的表達上調(diào)，提示Notch-1能增強OECs中NGF的合成。[關鍵詞]嗅球成鞘細胞；NGF；Notch-1[中圖法分類號]R741.05[文獻標志碼]ATheeffectofNotch-1onNGFexpressionofolfactoryensheathingcellsZ
4、huoFei,SunShanquan,YuWeihua,LiuHui,QiuGuoping,YangMei(DepartmentofAnatomy,InstituteofNeuroscience,ChongqingMedicalUniversity,Chongqing400016,China)[Abstract]ObjectiveObservetheeffectofNotch-1onNGFexpressionofolfactoryensheathingcells.MethodsActiveNotchplasm
5、id(NotchICV)，inactiveNotchplasmid(NotchVK)andemptyplasmidweretransfectedintoOECsofrats.Immunofluorescence、WesternblotandRT-PCRwereusedtodetecttheexpressionchangesofNGFanditsmRNA.ResultsImmunofluorescenceshowstheexpressionofNGFinNotchICVgroupwasnotablyincrea
6、sedcomparedwithNotchVKgroupandcontrolgroup(p<0.05);RT-PCRshowstheexpressionofNGFmRNAinNotchICVgroup(0.4961±0.0269)wasnotablyincreasedcomparedwithNotchVKgroup(0.2445±0.0134)andcontrolgroup(0.2459±0.0160)(P<0.01).WesternblotshowsthattheexpressionofNGFinNotchI
7、CVgroup(0.8875±0.0166)wasnotablyincreasedcomparedwithNotchVKgroup(0.6862±0.0592)andcontrolgroup(0.6821±0.0670)(P<0.01).ConclusionAllresultssuggestthatNotch-1couldimprovethesynthesisofNGFinOECs.[KeyWords]olfactoryensheathingcells;NGF;Notch-1SupportedbytheNat
8、ionalNaturalScienceFoundationofChina(30670743)andtheNationalNaturalScienceFoundationofChina(CSTC，2008BB5224).Correspondingauthor:SunShanquan,Tel:86-23-68485763,E-mail:sunsq2151@sohu.com編號201006168刊用形式論
