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1、編號：刊用形式：文章編號：IL-8/CXCR2軸促進兔內(nèi)皮祖細胞遷移、增殖及VEGF的表達毛梅1，2、呂學軍1、王藝1、徐劍鋮1，*（1第三軍醫(yī)大學新橋醫(yī)院呼吸病研究所重慶，400038；2廣州軍區(qū)武漢總醫(yī)院呼吸內(nèi)科武漢，430070）摘要目的觀察不同濃度白介素-8（interleukin-8，IL-8）對體外培養(yǎng)兔外周血內(nèi)皮祖細胞(endothelialprogenitorcells,EPCs)生物學功能的影響，探討趨化因子IL-8/CXCR2軸對EPCs血管生成功能的作用。方法密度梯度離心法獲取兔外周血單個核細胞(mononuclearcells,MNCs)，接種至纖
2、維連接蛋白(fibronectin,FN)包被的培養(yǎng)板上，培養(yǎng)7~10d后收集貼壁細胞鑒定EPCs，并用不同濃度IL-8的培養(yǎng)基繼續(xù)培養(yǎng)24h后采用MTT比色法、改良的Boyden小室實驗，觀察EPCs的遷移和增殖能力；采用RT-PCR、Western-blot方法檢測CXCR2、VEGF的mRNA及蛋白表達水平。結(jié)果從兔外周血成功分離EPCs細胞，其增殖、遷移和VEGF的分泌受IL-8調(diào)節(jié)，且隨著IL-8濃度升高而增強。結(jié)論IL-8/CXCR2軸能顯著促進EPCs的遷移、增殖及VEGF的表達。關(guān)鍵詞趨化因子；白介素-8；內(nèi)皮祖細胞中圖法分類文獻標識碼：ATheAxiso
3、fIL-8/CXCR2toPromoteProliferation,MigrationandVEGFExpressionofRabbits’EndothelialProgenitorCellsMAOMei1，2,LvXueJun1,Wangyi1,XUJian-cheng1，*(1,InstituteofHumanRespirationDiseases，XinqiaoHospital，ThirdMilitaryMedicalUniversity．Chongqing400038;2,DepartmentofRespiratoryMedicine,WuhanGeneralHo
4、spitalofGuangzhouCommand,Wuhan430070)Abstract:ObjectiveToinvestigatetheproliferation,migrationandsecretionbiologicalfunctionofrabbits’peripheralbloodendothelialprogenitorcells(EPCs)withdifferentconcentrationsofinterleukin-8.MethodsTotalmononuclearcells(MNCs)wereisolatedfromperipheralblood
5、bydensitygradientcentrifugation，andthenthecellswereplatedonfibronectin—coateculturedishes．Aftercultivationfor7days，attachedcellswereincubatedwithIL-8inaseriesofconcentrationsfor24h，thenattachedcellswerecharacterizedwithimmunofluorescenceandimmunohistochemistry．Theproliferationandmigration
6、activitiesofEPCswereassayedwithMTTassayandmodifiedBoydenchamberassay，andreversetranscription(RT)一polymerasechainreaction(PCR)andwestern-blotwereusedtoassessmRNAandproteinexpressionsofCXCR2andVEGFofEPCs.ResultsTheEPCsfromrabbits’peripheralbloodweresuccessfullyisolatedandidentified．Theirpro
7、liferation,migrationandsecretionfunctionswereregulatedbyIL-8,andenhancedwithincreasingconcentrationofIL-8.ConclusionTheIL-8/CXCR2axiscansignificantlypromotetheEPCsproliferation,migration,andVEGFexpression.Keywords：chemotacticfactor；interleukin-8；Endothelialprogenito
