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《缺氧缺血性腦損傷后豐富環(huán)境刺激對大鼠海馬ca1區(qū)神經(jīng)元凋亡的影響》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫。
1、缺氧缺血性腦損傷后豐富環(huán)境刺激對大鼠海馬CA1區(qū)神經(jīng)元凋亡的影響宋遠(yuǎn)見1，裴冬生1，孫亞峰1，張芳1，張兆成2，劉紅芝1（1徐州醫(yī)學(xué)院基礎(chǔ)學(xué)院，江蘇徐州221004；2徐州市中小學(xué)生衛(wèi)生保健所，徐州221002）提要：目的 觀察豐富環(huán)境刺激對缺氧缺血性腦損傷（hypoxic-ischemicbraindamage，HIBD）新生大鼠海馬CA1區(qū)神經(jīng)元的影響。方法 取健康7日齡SD大鼠90只，隨機(jī)分為3組:①正常對照組:30只,不建立模型,不刺激。②缺氧缺血非刺激組:30只,采用Sale法建立缺氧缺血性腦損傷模型,但不給豐富環(huán)境刺激。③豐富環(huán)境刺激組:30只,建立缺氧缺血性腦損傷模型,術(shù)后豐
2、富環(huán)境刺激(2h/次,1次/d)，共20d。分別在缺氧缺血后第3天和20天時各組隨機(jī)選10只，斷頭取腦，分別采用免疫印跡和焦油紫染色的方法檢測大鼠腦缺氧缺血后海馬CA1區(qū)JNK1/2和Bad(ser128)的磷酸化和神經(jīng)元凋亡情況。結(jié)果?、倥c正常對照組比較，缺血非刺激組腦缺氧缺血后第3天腦組織中JNK1/2和Bad(ser128)的磷酸化程度顯著增高(P＜0.05)；第20天海馬神經(jīng)元損傷非常嚴(yán)重(P＜0.05)。②豐富環(huán)境刺激組相對缺血非刺激組JNK1/2和Bad(ser128)磷酸化有所降低(P＜0.05)；海馬神經(jīng)元損傷明顯減輕(P＜0.05)。結(jié)論 豐富環(huán)境刺激可以促進(jìn)缺氧缺血性腦
3、損傷恢復(fù),減少海馬CA1區(qū)神經(jīng)元損傷，降低其中JNK1/2和Bad(ser128)的磷酸化程度是可能的機(jī)制之一。關(guān)鍵詞：缺氧缺血性腦損傷；豐富環(huán)境；海馬；JNK1/2；Bad(ser128)中圖法分類號：R72 文獻(xiàn)標(biāo)識碼：AEffectsofenrichedenvironmentalinterventiononcellsapoptosisofCA1regionduringhypoxia-ischemicbraindamageinrathippocampusSONGYuan-jian1,PEIDong-sheng1,SUNYa-feng1,ZHANGFang1,ZHANGZhao-che
4、ng2,LIUHong-zhi1(1SchoolofBasicMedicalSciencesofXuzhouMedicalCollege,Xuzhou221002,China;2HealthcareagencyofXuzhouprimaryandmiddleschoolstudents,Xuzhou221002,China)Abstract:ObjectiveToobservethetheenrichedenvironmentalinterventiononcellapoptosissinhippocampalCA1regionafterhypoxia-ischemicbraindamag
5、e(HIBD)inneonatalrats.Methods90healthySD7-day-oldratswererandomlydividedintothreegroups:①Controlgroup:30rats,nottobeestablishedhypoxic-ischemicbraininjurymodel,nottobestimulatedbyenrichedenvironment.②Non-stimulatedgroup:30rats,tobeestablishedmodelebutnottobestimulated.③Stimulatedgroup:30rats,tobee
6、stablishedmodelbeforeenrichedenvironmentalstimulation(2h/times,1/d)for20days.10ratswereselectedfromeachgrouponthethird3rddayandthetwentieth20thdayafterHypoxiaischemiaandthenthebrainsoweredecapitatedtodetectedthephosphorylationofJNK1/2andBad(ser128)inCA1regionbyWesternblotandneuronsinjurybyCreslyvi
7、olet-strained.Results①ThephosphorylationlevelofJNK1/2andBad(ser128)onthethird3rddayinnon-stimulatedgroupsignificantlyhigherincreasedthanincomparedwithcontrolgroup(P＜0.05);InjuryApoptosisofcellsOrcellloss?inhippoc
