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1、6Designingatwo-stagemolecularepidemiologystudy,peripheralbloodofcasesandcontrolswerecollected,thentheirDNAwereextracted,andMALDI-TOF-MSwasusedtoevaluateSNPsinthemiRNAsofinflammatorypathway.[Results]1WeusetransienttransfectiontechnologysuppresstheexpressionofXRCC1successfully
2、,andtheinhibitionefficiencyaftertransfectedin12h,24hand48hwere34.5%、64.4%and62.9%,respectively.2Thedifferencesamongthreegroupswerenotsignificantatthreetimepointswhenexpoursedtothedoseof2μg/ml.Expoursedto6μg/mlMC-LRfor16h,thedifferencebetweensiRNA-XRCC1groupandnormalcontrolgr
3、oupwassignificant.Andexpoursedto10μg/mlMC-LRfor8hor16h,thedifferencebetweensiRNA-XRCC1groupandtwocontrolgroupswassignificant.3Comparedwiththetwocontrolgroups,theexpressionofPARP-1ofsiRNA-XRCC1groupwasenhancedbyMC-LR(2μg/mlfor4h);ComparedwithsiRNA-XRCC1(2μg/mlfor4h),thelevels
4、oftheexpressionofPARP-1ofsiRNA-XRCC1(2μg/mlfor8h)andsiRNA-XRCC1(2μg/mlfor16h)werereduced;ThelevelofexpressionofPARP-1ofsiRNA-XRCC1(10μg/mlfor4h)islowerthansiRNA-XRCC1(2μg/mlfor4h).4Comparedwitheach0μg/mlgroup,theTailMomentdecreasedsignificantly.ThesiRNA-XRCC1groupcomparedwit
5、heithernormalgroupormissensesiRNAgroup,thedifferencesweresignificant,whichshowthatXRCC1knockeddowncellsweresensitiveforMC-LR.Andpositivecontrolexplainedthatthecometassaywassuccessful.5MultivariatelogisticregressionanalysisshowthathepatitisB(OR=164.799)anddrinkingditchpondwat
6、er(OR=8.750)wereriskfactorsoflivercancer,anddrinkingspringwater(OR=0.136),drinkingdeepwellwater(OR=0.028),drinkingtea(morethanonceaday)(OR=0.143)andeatingpickledfood(morethanonceaweek)(OR1=0.031,OR2=0.034,OR3=0.012)wereprotectivefactors.6Undercodominantmodel,carriersofrs2168
7、709GGgenotypehadastatisticallysignificantlyincreasedriskforlivercancer(OR=1.782,1.033-3.074);7carriersofrs11807848TCgenotypehadastatisticallysignificantlyincreasedriskforlivercancer(OR=1.622,1.088-2.418).Underdominantmodel,carriersofrs11807848TCorCCgenotypehadastatisticallys
8、ignificantlyincreasedriskforlivercancer(OR=1.665,1.138-2.436).Underrecessiv
