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1、暨南大學碩士研究生學位論文DNA指紋圖譜中，能得到更為全面、準確的藍細菌分布信息。因此，PCR-DGGE技術可作為傳統(tǒng)顯微觀察分析方法的有效補充，用于浮游植物群落結構分析。關鍵詞：微表層；次表層；浮游植物；PCR-DGGE；DNA指紋；大亞灣II暨南大學碩士研究生學位論文AbstractTheseasurfacemicrolayer(SML)isanimportantinterfacebetweentheatmosphereandthesea.Thisinterfacehasuniquephysicochemicalandbiologicalproperties.Anann
2、ualsurveywasconductedonthephytoplanktoncommunityandenvironmentalfactorsinDapengaoareaofDayaBaybetweenApril2013andMarch2014.Thepurposeofthisstudyistobetterunderstandphytoplanktoncomposition,quantitativedistributionandannualvariationofdominantspecies,andtoknowthecharacteristicsofphytoplankto
3、ncommunitystructureintheSMLandsubsurfacewater(SSW).Meanwhile,phytoplanktonDNAfingerprintswereanalyzedbyPCR-denaturinggradientgelelectrophoresis(PCR-DGGE)technique.TheresultsfromthisstudyenrichedtheinformationofphytoplanktonstructureintheSMLofDayaBay,andprovidedascientificbasisforenvironmen
4、talprotectioninthisseaarea.Atotalof142taxawereidentifiedduringthesurvey,including132speciesfromtheSMLand128fromtheSSW.However,theShannon-Weaverspeciesdiversityindexandevennesswaslow,indicatingthedeteriorationofenvironmentsinDayaBayintherecentyears.Diatomwasthepredominantgroupofphytoplankto
5、n,followedbydinoflagellate,andfewoccurrenceofspeciesinothergroupssuchascyanobateria,chrysophytes，cryptophytesandrhaphidophytes.ThecommonpredominantspeciesincludedChaetoceros,Skeletonemacostatum,Pseudo-nitzschiaandLeptocylindrusdanicus,whichwerepresentedthroughoutthewholeyear.Meanwhile,Rhiz
6、osoleniafragilissima,Cerataulinapelagica,Guinardiastriatadominatedinsomeparticularmonths.Thephytoplanktonabundancewashighaswell,withtheannualaveragecellsdensityof662.07×10cells/Land1.48×10cells/LintheSMLandSSW,respectively.Thecelldensitypeakedinsummer,followedbywinter,andwaslowerinspringan
7、dautumn.AhighpeakofS.costatumoccurredinFebruary,whichresultedinwinterpeakofphytoplankton.ThephytoplanktonstructureintheSMLwassimilartothatintheSSW,andwascharacterizedbythedominanceofdiatoms,highspeciesrichnessandlowspeciesdiversity.Thetotalphytoplankton,diatom
