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1、獨創(chuàng)性聲明秉承學(xué)校嚴(yán)謹(jǐn)?shù)膶W(xué)風(fēng)與優(yōu)良的科學(xué)道德，本人聲明所呈交的論文是我個人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知，除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，不包含本人或他人已申請學(xué)位或其他用途使用過的成果。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示了致謝。申請學(xué)位論文與資料若有不實之處，本人承擔(dān)一切相關(guān)責(zé)任。論文作者簽名：日期：保護(hù)知識產(chǎn)權(quán)聲明本人完全了解第四軍醫(yī)大學(xué)有關(guān)保護(hù)知識產(chǎn)權(quán)的規(guī)定，即：研究生在校攻讀學(xué)位期間論文工作的知
2、識產(chǎn)權(quán)單位為第四軍醫(yī)大學(xué)。本人保證畢業(yè)離校后，發(fā)表論文等使用本論文工作成果時第一署名單位仍然為第四軍醫(yī)大學(xué)。學(xué)?？梢怨颊撐牡娜炕虿糠謨?nèi)容（含電子版，保密內(nèi)容除外），可以采用影印，縮印或其他復(fù)制手段保存論文；學(xué)校有權(quán)允許論文被查閱和借閱，并在校園網(wǎng)上提供論文內(nèi)容的瀏覽和下載服務(wù)。同意學(xué)校將論文加入《中國優(yōu)秀博碩士學(xué)位論文全文數(shù)據(jù)庫》和編入《中國知識資源總庫》等，并可瀏覽和下載，同時享受《中國優(yōu)秀博碩士學(xué)位論文全文數(shù)據(jù)庫出版章程》規(guī)定的相關(guān)權(quán)益。論文作者簽名：導(dǎo)師簽名：日期：第四軍醫(yī)大學(xué)碩士學(xué)位論文目錄縮略語表
3、·······························································································1中文摘要·······························································································3英文摘要··························································
4、·····································6前言·······························································································9文獻(xiàn)回顧······························································································10正文····················
5、··········································································31第一部分：c-Met在肝癌細(xì)胞中的表達(dá)及其下游通路的鑒定··························31引言·······························································································311材料························
6、····································································311.1實驗細(xì)胞················································································311.2主要試劑················································································321.3主要儀器···
7、·············································································332方法····························································································342.1細(xì)胞培養(yǎng)及傳代·······································································3
8、42.2Real-timePCR檢測····································································342.3細(xì)胞免疫熒光檢測····································································352.4Western-blot檢測········
