兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化

兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化

ID:32361212

大?。?.02 MB

頁數(shù):4頁

時(shí)間:2019-02-03

兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化_第1頁
兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化_第2頁
兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化_第3頁
兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化_第4頁
資源描述:

《兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在工程資料-天天文庫。

1、中國組織工程研究與臨床康復(fù)第14卷第6期2010–02–05出版JournalofClinicalRehabilitativeTissueEngineeringResearchFebruary5,2010Vol.14,No.6兔骨髓間充質(zhì)干細(xì)胞體外分離培養(yǎng)及多向誘導(dǎo)分化*★徐成峰,胡大海,趙周婷,張萬福,白曉智,蔡維霞Isolation,cultureandmultipledifferentiationsofrabbitbonemarrow-derivedmesenchymalstemcellsinvitroXuCheng-feng,HuDa-hai,ZhaoZhou-ting

2、,ZhangWan-fu,BaiXiao-zhi,CaiWei-xiaAbstractDepartmentofBurnsandCutaneousBACKGROUND:Mesenchymalstemcells(MSCs),withlowimmunogenicity,canregulatecellularimmunityandmitigategraftSurgery,Burnsrejection,whichhasagoodprospectintissueengineering.However,itisrarelypresentinbonemarrow.CenterofPLA,Xij

3、ingOBJECTIVE:Toexploreanisolationandculturemethodoftherabbitbonemarrow-derivedMSCs,toobservethebiologicalHospital,theFourthMilitaryMedicalcharacteristicsanddifferentiationpotentialofbonemarrow-derivedMSCs.UniversityofChineseMETHODS:MSCswereisolatedfromrabbittibiabonemarrowbycombinationofgrad

4、ientcentrifugationanddifferentPLA,Xi’an710032,adherentmethod,thenproliferationinvitro.Morphologywasexaminedbyphasecontrastmicroscopy,andthegrowthcurveShaanxiProvince,ofculturedMSCswasdrawnviaMTTresults.MSCsweretreatedwithosteogeneticinductor(L-DMEM/F12,10%fetalbovineChinaserum,0.1μmol/Ldexam

5、ethasone,200μmol/LvitaminC,10mmol/Lβ-phosphoglycerol),adiposeinductor(L-DMEM/F12,10%XuCheng-feng★,FBS,1μmol/Ldexamethasone,200μmol/Lantifani,0.5mmol/LIBMX,10μg/mLinsulin),andchondrocytesinductorStudyingformaster’s(L-DMEM/F12,10%FBS,10μg/LTGF-β1,0.1μmol/Ldexamethasone,50μmol/LvitaminC,6.25mg/

6、Linsulin)todifferentiateddegree,Physician,intoosteoblast,dipocytesandchondrocytes.Andthedifferentiatedcellswereidentifiedbyalkalinephosphatasestaining,oilredDepartmentofBurnsandCutaneousOstaining,andtoluidinebluestaining,respectively.Surgery,BurnsRESULTSANDCONCLUSION:Bonemarrow-derivedMSCsca

7、nbeisolatedandculturedbythecombinationofgradientCenterofPLA,Xijingcentrifugationanddifferentadherentmethodinvitro,whichhavethebetterpotentialityofproliferationandmulti-directionalHospital,theFourthdifferentiation.Mostlyoftheprimaryandpassagedcellsw

當(dāng)前文檔最多預(yù)覽五頁,下載文檔查看全文

此文檔下載收益歸作者所有

當(dāng)前文檔最多預(yù)覽五頁,下載文檔查看全文
溫馨提示:
1. 部分包含數(shù)學(xué)公式或PPT動(dòng)畫的文件,查看預(yù)覽時(shí)可能會(huì)顯示錯(cuò)亂或異常,文件下載后無此問題,請(qǐng)放心下載。
2. 本文檔由用戶上傳,版權(quán)歸屬用戶,天天文庫負(fù)責(zé)整理代發(fā)布。如果您對(duì)本文檔版權(quán)有爭議請(qǐng)及時(shí)聯(lián)系客服。
3. 下載前請(qǐng)仔細(xì)閱讀文檔內(nèi)容,確認(rèn)文檔內(nèi)容符合您的需求后進(jìn)行下載,若出現(xiàn)內(nèi)容與標(biāo)題不符可向本站投訴處理。
4. 下載文檔時(shí)可能由于網(wǎng)絡(luò)波動(dòng)等原因無法下載或下載錯(cuò)誤,付費(fèi)完成后未能成功下載的用戶請(qǐng)聯(lián)系客服處理。