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1、分類號(hào)S641.3學(xué)校代碼10129UDC633學(xué)號(hào)2009202062嫁接對(duì)溫度脅迫下辣椒抗性生理指標(biāo)的影響研究Effectofgraftingtopepperstressresistancephysiologicalindexesundertemperaturestress申請(qǐng)人:王水霞學(xué)科門類:農(nóng)學(xué)學(xué)科專業(yè):蔬菜學(xué)研究方向:設(shè)施蔬菜栽培指導(dǎo)教師:崔世茂教授論文提交日期:二〇一二年六月摘要本試驗(yàn)選用野生辣椒品種“威壯貝爾”作砧木,分別在光照培養(yǎng)箱和溫室中研究了辣椒及其嫁接后低溫對(duì)其苗期葉片、根系和高溫對(duì)其結(jié)果期葉片生理指標(biāo)的影響,明確嫁接對(duì)辣椒抗溫度脅迫的影響,旨在為辣椒耐寒、
2、耐熱性栽培及生產(chǎn)提供依據(jù)。結(jié)果表明:1.低溫逆境下,較自根苗相比嫁接可降低辣椒葉片和根系MDA含量及相對(duì)電導(dǎo)率,減緩超氧陰離子產(chǎn)生速率,從而降低低溫下辣椒的膜質(zhì)過(guò)氧化程度,減輕對(duì)細(xì)胞膜的傷害。2.低溫逆境下,較自根苗相比嫁接還可提高辣椒葉片和根系中抗氧化保護(hù)酶類(SOD、POD、CAT)的活性及滲透調(diào)節(jié)物質(zhì)(游離脯氨酸、可溶性蛋白、可溶性糖)含量且比自根苗多保持2d左右的高抗氧化保護(hù)酶活性和高滲透調(diào)節(jié)物質(zhì)含量。3.綜合辣椒幼苗細(xì)胞膜受傷害程度、抗氧化酶類活性、滲透調(diào)節(jié)物質(zhì)含量方面的研究,可以得出,嫁接可有效的提高辣椒苗期抗冷的能力,延長(zhǎng)辣椒抵御苗期低溫(晝夜溫度為10℃和5℃)2d
3、左右。4.高溫逆境下,較自根苗相比嫁接可減輕辣椒葉片細(xì)胞膜的受損害程度,提高抗氧化保護(hù)酶類的活性和滲透調(diào)節(jié)物質(zhì)的含量,說(shuō)明嫁接可以有效地提高辣椒抵抗熱脅迫的能力。關(guān)鍵詞:嫁接;辣椒;溫度脅迫;生理指標(biāo)EffectofgraftingtopepperstressresistancephysiologicalindexesundertemperaturestressAbstractThisstudyselectedthepeppervarieties“VeryStrongBell”asgraftedrootstock.Respectively,inlightofincubatorand
4、greenhouse,theeffectoflowtemperaturestressonpepperseedingleaves,rootsphysiologicalindicatorsandhightemperaturestressonpepperfruitingleavesphysiologicalindicatorsofself-rootedandgraftedpepperwereresearched.CleartheeffectofgraftingonPepperResistancetotemperaturestress,soastoprovidethebasisforpep
5、percultivationandproductiontheunderCold,heatstress.resultsshowthat:1Underlowtemperaturestress,comparedwithself-rootedpepper,graftingcanreducetheMDAcontentandtherelativeconductivity,canslowdowntheproductionrate-of02ofpepperseedlingleavesandroots,therebycanreducingmembranelipidperoxidationandred
6、ucingdamagetothecellmembraneofthepepperseedling.2Underlowtemperaturestress,comparedwithself-rootedpepper,graftingcanalsoimprovetheactivityofprotectiveantioxidantenzymes(SOD,POD,CAT)andthecontentofpenetrationregulatingsubstances(freeproline,solubleprotein,solublesugar)ofpepperseedlingleavesandr
7、oots.graftedpeppercankeephigherprotectiveantioxidantenzymeactivityandpenetrationregulatingsubstancescontentabout2dthanself-rootedpepper.3Comprehensivelyanalysisthedegreeofthecellmembraneinjury,theactivityofantioxidantenzymesandtheconten