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1、有9.2ram。BC32對葉的抑制作用最好,在前期對莖上病斑的抑制作用較強(qiáng),但在后期就有所下降。但在防治試驗(yàn)中,YJl的防治效果明顯高于其它菌的田間防效。5.放線菌的定殖I-ID8和CS4都能在土壤內(nèi)短期定殖,在第40d時(shí),數(shù)量級分別為107和104,而且數(shù)量還有下降的趨勢。而YJl和BC32在土壤中的數(shù)量一直在增長,40d時(shí)還有大量生長的趨勢。放線菌在葉上的定殖較弱,HD8和CS4在接種6"---'9d以后,在葉上不能檢測到標(biāo)記菌。BC32和YJl在葉上存在的時(shí)間較長,但數(shù)量也是很快在減少。所有放線菌都能在油菜苗內(nèi)定殖,在油菜內(nèi)的數(shù)量都是先增加后減少。其中,在根部的定殖數(shù)量最多。灌根處理以
2、后,YJl在第45d的時(shí)根內(nèi)數(shù)量達(dá)2.312x105efu/g。其余3種放線菌在油菜內(nèi)的定殖數(shù)量都是根>莖>葉,根內(nèi)的標(biāo)記放線菌數(shù)量大于莖內(nèi)10,--。102個(gè)數(shù)量級。使用灌根和浸根的方法都能使放線菌定殖,’但是灌根處理比浸根處理的放線菌數(shù)量要多。關(guān)鍵詞:油菜菌核病菌;生物防治;放線菌;拮抗;定殖II,,StudyontheBiocontroiofSclerotiniasclerotiorumbyAntagonisticActinomycetesYa0Jia(phytopathology)DirectedbyProf.HuangYunSclerofiumwiththecharacterist
3、icofsoil·borneandaerobom'ediseaseWasoneoftheimportantrapedisease.Thehigherresistantactinomycetestrainswerescreenedfromrhizospheresoilandendophyticactinomycetesofchinesecabbage,Camelliasinensis,OrmosiahosieiandG加枷bilob.Meanwhile,theinfluenceofrapeseedlingresistancebyusingantagonisticactinomyces.Thec
4、oloniza-tionofactinomycesinsoil,hylloplaneofrape,andrapeseedingWasstudied.And,indoorpot,leavesinvitroandfieldtestsprovidedtheoreticalevidencefordeveloppingmicrobialmanure.1.Isolationandscreeningofantagonisticaetinomyces165actinomycetestrainswereseparatedfromrhizospheresoilandendophyticactinomycetes
5、ofchinesecabbage,Camelliasinensis,OrmosiahosieiandGinkgobilob.25strainsoftheisolateswerefoundsuppressingScleotiniasclerotiorummecyliumgrowthbypairingculgare.Amongthem,YJl,BC32,HD8andCS4withhigherresistancewerefound.YJlwasfromthebranchofGinkgobilob,andtheotherthreewerefromsoil.Theinhibitionzonewideo
6、fYJl,BC32,HD8andCS4were11,1l,8and10mm.Inthedualplateassays,andtheanti—actinomyeeteseffectrespectivelyreached73.5%、84.4%、58.1%and50.7%Thegerminationofsclerotiumandthecasporouswereinhibitedinhi.曲一concentrationactinomyceteliquid,buttheantibacterialeffectdecreasedinlow-concentrationactinomyceteliquid.2
7、.IdentificationofaetinomycetestrainsYJl,BC32,HD8andCS4wereclassifiedasStreptomycesbymorphologicalcharacters,culturalcharacters,physiologicalbiochemicalcharactersand16SrDNAsequenceanalysis.3·Rapeseedlingresi