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1、農(nóng)業(yè)大學(xué)學(xué)報1998,21(3):76～79rnalofNanjingAgriculturalUniversityX不同濃度海南霉素對山羊瘤胃消化代謝的影響任明強(qiáng)沈贊明趙茹茜陳杰(南京農(nóng)業(yè)大學(xué)農(nóng)業(yè)部動物生理生化重點開放實驗室,南京210095)摘要試驗動物為3頭裝有永久性瘤胃瘺管的閹公山羊。試驗分為4期:對照、試驗é、ê和?,每期10d。用海南霉素乙醇溶液灌入瘤胃,劑量分別為0.0000,0.0025,0.0100和0.0500mg?(kg·d)。適應(yīng)8d后從8:00到20:00每3h由瘤胃瘺管采集瘤胃液。試驗發(fā)
2、現(xiàn),3個試驗期瘤胃液中乙酸與丙酸之比(A?P)和NH32N濃度明顯下降,并呈一定的劑量效應(yīng)。試驗ê和?A?P分別比對照期下降5.62%(P<0.05)和13.30%(P<0.01),NH32N濃度分別下降17.96%(P<0.05)和33.80%(P<0.01);試驗é相應(yīng)指標(biāo)雖有下降,但差異不顯著。與對照期相比,各組總揮發(fā)性脂肪酸(TVFA)均無明顯改變,微生物蛋白(MCP)除試驗é外亦無明顯變化。結(jié)果表明:海南霉素可改變瘤胃發(fā)酵類型,明顯提高丙酸比例,抑制飼料蛋白在瘤胃內(nèi)的降解。關(guān)鍵詞山羊;瘤胃消化;代謝;離
3、子載體;海南霉素;揮發(fā)性脂肪酸;氨氮;瘤胃微生物中圖分類號S82711EffectofionophoreHainanmycinatdifferentconcentrationonrumenmetabolismofgoatsRenMingqiang,ShenZanming,ZhaoRuqianandChenJie(KeyLabofAnimalPhysiology&Biochemistry,MinistryofAgriculture,NanjingAgricUniv,Nanjing210095)ABSTRACTThr
4、eecastratedmalegoatswithcannulaeintherumenwereused.Theexperimentwasdi2videdintofoursuccessiveperiods,i.e.controlandexperimenté,êand?,eachlasted10days.Incon2trolandexperimenté,êand?,theHainanmycindissolvedinsolutionofethanolwasinfusedthroughruminalcannulaeatad
5、osageof0.0000,0.0025,0.0100,0.0500mg?(kg·d)respectively.Rumencontentsamplesweretakenthroughtheremencannulaeat0,3,6,9,12hafterthemorningfeedingond29andd210ofeachperiod.Inthreeexperimentperiods,theratioofacetatetopropionate(A?P)andtheconcentrationofammonianitro
6、geninrumenweredecreasedandtherangedeclinedwerepositivelyrelatedwiththedosageofinfusedHainanmycin.Inexperimentêand?,comparingtocontrol,theA?Pwasde2clined5.62%(P<0.05)and13.30%(P<0.01),theconcentrationofammonianitrogenwasreduced17.96%(P<0.05)and33.80%(P<0.01)re
7、spectively,whiletheaboveparameterswerenotaffectedmarkedlyinexperimenté.Comparetocontrol,Hainanmycindidnotaffecttotalvolatilefattyacidcon2centrationinrumenandthemicrobialproteinwasnotfoundvariedsignificantly,exceptexperimenté.TheresultssuggestthatHainanmycinim
8、proverumenfermentationandreducetherumendegradationofdi2etaryprotein.Keywordsgoat;ruminaldigestion;rumenmetabolism;ionophore;Hainanmycin;volatilefattyacid;X3農(nóng)業(yè)部重點資助項目(95牧202201)收稿日期:199721
