牙齦卟啉單胞菌rag位點(diǎn)基因分型與慢性牙周炎的相關(guān)性分析

《牙齦卟啉單胞菌rag位點(diǎn)基因分型與慢性牙周炎的相關(guān)性分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、>0．05)。rag-1，3型在中重度組的聯(lián)合檢出率高于輕度組(尸O．05)。3、rag-1基因在不同牙周炎癥狀態(tài)-FRNA表達(dá)檢測(cè)結(jié)果輕度組、中度組和重度組酏Jrag-1型基因?qū)η磉_(dá)水平分別顯示增強(qiáng)，減弱和增強(qiáng)。輕度組和重度組rag-1型基因的RNA表達(dá)水平與牙周炎癥狀態(tài)呈正相關(guān)，而與中度組則呈負(fù)相關(guān)。結(jié)論1、不同，．昭基因型的尸gingivalis在慢性牙周炎患者齦下菌斑中的分布有所不同。rag-1型的檢出率最高，其次為rag-3型，表BJ目rag-1型和，．紛3型基因與我國(guó)人群慢性牙周炎的發(fā)生發(fā)展密切相關(guān)。ra

2、g-1型在中重度組的檢出率高于輕度組，表明攜帶rag-1型基因的戶gingivalis更能造成牙周組織嚴(yán)重的破壞，與中重度慢性牙周炎密切相關(guān)。2、rag-1型基因在輕度組、中度組和重度組的RNA表達(dá)水平分別呈現(xiàn)增高．降低一增高的趨勢(shì)，表BJgrag-1型基因的RNA表達(dá)水平隨著牙周組織破壞程度及炎癥狀態(tài)的不同而改變。關(guān)鍵詞慢性牙周炎；牙齦卟啉單胞菌；rag；基因型3·英文論著摘要·TheCorrelationAnalysisoftheragLocusGenotypesofP,"phjgingivalisandChronicPeriodontitisoIt"orpltyromona

3、sgmgivalisandhroniceriodontitisobjectiveTheaimofthepresentstudieswastodetectthedispositionofPorphyromonasgingivalis(P．gingivalis)offourdifferentraglocusgenotypes，andtodetecttheexpressionofrag-1inRNAlevelindifferentperiodontalinflamedconditionsbypolymerasechainreaction(PCR)andreversetranscript

4、ion—polymerasechainreaction(RT-PCR)．WediscussedthedispositionregularityoftheraglocusgenotypesofPorphyromonasgingivalis，andtheexpressionregularityofrag-1inRNAlevelindifferentperiodontalinflamedconditions．Methods1．StudysubjectsAtotalof50subjectswereenrolledinthestudieswhowereselectedfromtheperi

5、odontaldepartmentofSchoolofStomatology，ChinaMedicalUniversity．Subjectswereexcludedfromthestudiesifanyofthefollowingconditionsapplied：systemicdisease，pregnancy，professionaltoothcleaning，periodontaltherapyduring1yearortakinganymedicineduring3monthsprecedingtothestudy．Allsubjectsenrolledintothes

6、tudiessignedtheinformedconsentform．2．MeasurementofclinicalperiodontalconditionWeselectedthreelesionstatusesinchronicperiodontitissubjects．ClinicalparametersincludedProbingDepth(PD)、clinicalattachmentloss(CAL)andthelossofalveolarbonebyX-ray．Alltheclinicalparametersweremeasuredandrecordedbyasin

7、gleexaminer．43．SamplescollectionSubgingivalplaquesampleswereoNainedwithsteriletoothpicksintolmlofPBSforDNAandRNAisolation．4．SamplesgroupingThesamplesof50subjectsweredivideintotwogroups，whichwerelightgroupandmoderately-severelygroupaccordingto