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《hif-1α與vegf在大鼠缺血缺氧性腦損傷中的表達(dá)分析》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、中文摘要3HIF．1a只在一定氧濃度范圍內(nèi)表達(dá)，梗塞中心區(qū)完全缺氧情況下，神經(jīng)元壞死后則無(wú)表達(dá)；缺血半暗區(qū)存在一定程度缺氧，HIF．1a在此處的神經(jīng)元中存在明顯高表達(dá)。4HIF．1a與VEGF于缺血梗塞24h時(shí)蛋白表達(dá)水平達(dá)到高峰，隨梗塞時(shí)間的延長(zhǎng)其表達(dá)水平呈峰樣變化。5在缺血梗塞后，HIF．1a與VEGF在腦組織中的表達(dá)水平隨時(shí)間變化表現(xiàn)出升降一致的特點(diǎn)，二者的表達(dá)之間具有明顯的相關(guān)性。關(guān)鍵詞：動(dòng)物模型；腦缺血；缺氧誘導(dǎo)因子．10【；血管內(nèi)皮生長(zhǎng)因子英文摘要一_一—————————_—————————_—^————————————————————————————
2、———————_————一TheexperimentalstudyonexpressionofHIF··laandVEGFinratswithhypoxic--ischemicbraindamageABSTRACTCerebralStrokeisaverycommonfataldiseaseinnervoussystem．Itthreatenshuman’Shealthwithitshighattackrate，disabilityrateandcasefatalityrate．AndischemicCerebralStrokeoccupiesabout60．80
3、％ofthetotal．Sometherapiesincludethrombolysisandcraniotomyareeffectivetofewpatientsatpresent．Therefore，it’Simminenttoilluminatethemolecularmechanismandfindanewtherapeuticregimen．objective：Thisexperimentalstudyisforpreparingaratmodelwithpermanentmiddlecerebralarteryocclusion(MCAO)andest
4、imatingthemodelbyusingthefollowingmethodsas：ethologyscoreofthelossofneuralfunction，determiningthe‘nfaretithroughTTCstaininobservingtheinfarctionscopetlarougnstaining,oDserVmghistopathologyandultramicrostructureoftherat’sbraintissues，andforfindingthetheoryevidenceofeffectivetreatmentby
5、detectingtheexpressionofHIF—laandVEGFintheexperimentalischemicbraintissuesandobservingtheregularityofexpressionandmolecularmechanism．Methods：lAnimalsandgroups：Atotalof80healthyadultSprague．Dawleyrats，weighting250—2809，randomlydivided5英文摘要into3groups：normalcontrolgroup(n=5)，experimentg
6、roup(n-50)，andshamoperatedgroup(n=25)．Theexperimentgroupandtheshamoperatedgroupdividedinto5subgroupsbydifferentinfarctiontime：6h，12h，24h，72h，7d．2Modelpreparation：Preparetheratmodelwithpermanentmiddlecerebralarteryocclusion(MCAO)usingintraluminalsuturebyZeaLonga’Smethod．Theoperationste
7、pofshamoperatedgroupwassameasexperimentgroupexceptforpluggingthesutureincarotidartery．Allratswereestimatedbyethologyscoreofthelossofneuralfunctionafteroperation．Theanimalswhosescorefrom1-3wereselectedtoexperimentgroup．3Animalsineachgroupwerekilledatdifferentpresettime，andthebrainswere
8、colle
