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1、萬方數(shù)據(jù)華中農(nóng)業(yè)大學(xué)2015屆碩士研究生學(xué)位論文mg／L)中，兩者的值又有所回升。4．低氧對(duì)肝臟抗氧指標(biāo)的影響對(duì)不同低氧組團(tuán)頭魴肝臟各種抗氧化酶指標(biāo)的檢測(cè)結(jié)果顯示，低氧會(huì)導(dǎo)致肝臟中SOD和CAT酶活性、過氧化氫(H202)降低。肝臟中一氧化氮(NO)水平隨溶氧濃度的降低及低氧處理時(shí)間的延長逐漸增加。肝臟總ROS水平隨著低氧處理時(shí)間的延長逐漸降低，而復(fù)氧及嚴(yán)重低氧時(shí)ROS含量回升。關(guān)鍵詞：團(tuán)頭魴；低氧；細(xì)胞凋亡；心臟；TUNEL萬方數(shù)據(jù)低氧對(duì)團(tuán)頭魴細(xì)胞凋亡及抗氧化酶活性的影響AbstractMegalobramaamb
2、lycephala,alsocalledWuchangfish,isakindofimprovedeconomicfreshwaterfish．Inrecentyears，withthedeteriorationofnaturalenvironmentandhumanactivityintervention,itslivingenvironmentoftenappearedlowoxygen,restrictingthedevelopmentof彪amblycephalabreeding．Inourstudy,the
3、structurechangesofdifferenttissuesofMamblycephalawereobservedafterhypoxiatreatment,andtheactivitiesofantioxidantenzymeswereanalyzedintheliverinhypoxiaandhypoxia-recoveryconditions．Theinfluenceoflowoxygenonmyocardialcellapoptosisandbcl-2expressionwerealsostudied
4、byTUNELandfluorescencequantitativePCR．Additionally,thechangesofMDAcontentandSODactivitywereanalyzedinheartmitochondriaextractedusingsucrosegradientcentrifugationmethod．Themainresultswereasfollows：1．Effectsofhypoxiaonstnl曲lreofdifferenttissuesofM．a(chǎn)mblycephalaDif
5、ferenttissues(heart，liver,kidney,muscle，spleen)weresampledandobservedbasedonHEstainingafterseverehypoxictreatment(DO：0．5mg／L)，andtheresultsrevealedthatgreaterinfluenceofhypoxiaontheheartandkidney,whichmusclefmersinheartshowedswellinganddisorderinarrangementinse
6、verehypoxiaconditions，brokencapillarieswereobservedinspleen．However,hypoxiae虢ctsonstructureofmuscle，liverspleenwererelativelylesser．Theresultsshowedbasedonelectronmicroscopethatundermoderatehypoxia(DO：2．5+0．5mgmfor2h，4”，myocardialmitochondrialstructurewerebroke
7、n，mitochondriaWasswellinganddisorder,butinserverhypoxia(DO：O．5mg／L)，somemitochondriawerefractured．2．CardiaccellapoptosiscausedbyhypoxiaInordertofurtherstudyhypoxiae毹ctsonhearttissueofMamblycephala，cardiaccellapoptosisandbcl-2mRNAlevelswereanalyzedbyTUNEL(termin
8、aldexynucleotidyltransferaseTdTmediateddUTPNickendlabeling)andfluorescencequantitativePCRafterhypoxiatreatment．Theresultsshowedthathypoxiainducedcardiaccellapoptosis，andwith
