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1、廣林九號桜葉精油的抑菌活性研究作者：m玉紅周琪頗俊華孟陸麗李梓來源：《湖北農(nóng)業(yè)科學(xué)》2013年笫21期摘要:研究了丿林九號^［EucalyptusurophyllaxEucalyptusgrandis(Guanglin9)］葉精油的抑菌活性。利用氣相色譜■質(zhì)譜聯(lián)用技術(shù)分析桜葉精油的化學(xué)成分，采用濾紙片法評價(jià)其抑菌活性，以平板稀釋法測定了粘:油的最小抑菌濃度。廣林九號桜葉粘油的主要成分有：1,8■桜葉油素(40.844%)、a■兼烯(24.221%)、a■松油醇(4.661%)、反式?松香芹醇(3.735%)、龍腦(2.762%
2、)、乙酸松油脂(3.592%)和松香芹酮(1.978%)。廣林九號桜葉精油對測試的菌種均有良好的抑菌活性，但對細(xì)菌和霉菌的抑菌效果有所差異。對金黃色葡萄球菌(Staphylococusaureus)、枯草芽抱桿菌(Bacillussubtilis)、大腸桿菌(Escherichiacoli),黃曲霉(Aspergillusflavus)、黑曲霉(Aspergillusniger)和橘青霉(Penicillumcitrinum)的最小抑菌濃度分別為10、20、20、10、10、5mL/L。關(guān)鍵詞：廣林九號桜［Eucalyptus
3、UrophyllaxEucalyptusGrandis(Guanglin9)］葉；精油;抑菌活性；最小抑菌濃度中圖分類號:TQ654+.2文獻(xiàn)標(biāo)識碼：A文章編號:0439-8114(2013)21-5193-03StudiesonAntimicrobialActivityofEssentialOilfromLeavesofEucalyptusurophy11axEucalyptusgrandis(Guanglin9)TIANYu-hongl,ZHOUQ訂，POJun-hua2,MENGLu-lil,LIZ訂(1?Departm
4、entofBiologicalandChemicalEngineering,GuangxiUniversityofTechnology,Liuzhou545006,Guangxi,China；2.LiuzhouHualiHouseholdProductsLimitedCompany,Liuzhou545006,Guangxi,China)Abstract：TheantimicrobialactivityoftheessentialoilfromleavesofEucalyptusurophyllaxEucalyptusgran
5、dis(Guanglin9)wasstudied.Theessentialoilwasanalyzedbygaschromatography-massspectrometry(GC-MS)method.Theantimicrobialactivityofoilwasevaluatedbyusingfilterpapermethodandtheminimuminhibitoryconcentration(MIC)wasdeterminedbyplatedilutionmethod?Themaincomponentsofthees
6、senti-aloilwere1,8-cineole(40.844%),followedbya-pinene(24.221%),a-terpineol(4.661%),a-terpinylacetate(3.592%),borneol(2.762%),trans-pinocarveol(3.735%)andpinocarvone(1.978%).Theresultsindicatedthattheessentialoilhadexcellentantimicrobialactivityagainsttestedmicroorg
7、anisms.Theeffectsofessentialoilonantibacterialandantifiingalactivitiesdifferedobviously.Theminimuminhibitoryconcentrations(MICs)were10,20,20,10,10and5mL/LforStaphylococusaureus,Bacillussubtilis,Escherichiacoli,Aspergillusflavus,AspergillusnigerandPenicillumcitrinum,
8、respectively.Keywords：EucalyptusurophyllaxEucalyptusgrandis(Guanglin9)；essentialoil；antimicrobialactivity；minimuminhibitoryconcentration廣譏
