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1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文盧氏綠殼蛋雞蛋殼腺中膽綠素時(shí)空變化及雞蛋品質(zhì)的變化姓名：張學(xué)武申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：李奎；康相濤201206河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院2012屆畢業(yè)研究生摘要本文運(yùn)用人體解剖學(xué)、組織化學(xué)技術(shù)、分子生物學(xué)測(cè)定22．31周齡盧氏綠殼蛋雞雞蛋殼中膽綠素含量、雞蛋品質(zhì)、殼腺部絨毛高度、殼腺部膽綠素含量、殼腺部血紅素加氧酶．1的mRNA相對(duì)表達(dá)量，米研究不同周齡盧氏綠殼蛋雞殼腺部膽綠素時(shí)空變化與蛋品質(zhì)的關(guān)系，為盧氏綠殼蛋雞的生產(chǎn)養(yǎng)殖提供基礎(chǔ)理論。結(jié)果顯示：(1)盧氏綠殼蛋雞綠殼蛋蛋殼中H日綠素含量先降低斤增加再降低的波動(dòng)變化。第22周蟹殼中膽綠
2、素含量高丁第25周，著異顯著。第28周月日-綠素含量高丁第25周，籌異顯著。(2)在22—31周齡，盧氏綠殼雞蛋品質(zhì)隨著周齡的增加而增人。蛋黃重逐漸增加，差異顯著。(3)在22-31周齡，盧氏盧氏綠殼蛋雞殼腺部絨毛高度逐漸增加，第22周與第25周、28周、31周著異顯著。(4)盧氏綠殼蛋雞殼腺部膽綠素含量在第22周至第31周，殼腺部膽綠素含量逐漸增加，第22周與第25周、第28周、第31周著異顯著。(5)盧氏綠殼蛋雞殼腺部血．紅素加氧酶．1的mRNA相對(duì)表達(dá)量是先增加后減少再穩(wěn)定的變化趨勢(shì)，第25周血．紅素加氧酶．1的mRNA相對(duì)表達(dá)量高?。?2周、第28周、第31周，籌異顯
3、著。關(guān)鍵詞：盧氏純綠殼蛋雞；殼腺部；膽綠素；雞蛋品質(zhì)；血紅素加氧酶．1ThespatialtemporalchangesofbiliverdinintheshellglandandthechangesofeggqualityofGreen—EggshellofLushiChickenABSTRACT：TostudytherelationshipbetweenthespatialtemporalchangesofbiliverdinintheshellglandandtheeggqualityofGreen—EggshellofLushiChickenandDrovidetheo
4、riesbasisforproductionandfarmingofLushiGreen。EggshellChicken．weusedtheanatomytechnology、histochemistrytechnology、Molecularbi010堅(jiān)vtecllniquestodetectethebiliverdincontentingreeneggshell、Eggquality、villiheightofgreenshellhenseggshellgland、Thebiliverdincontentofgreenshellhenseggshellgland、theHO
5、一1mRNARelativeexpressionineggshellglandofgreenegg．shellofLushiChickenin22weeksto31weeks．Theresultsshowedthat：(1)ThebiliverdincontentofGreenshelleggseggshellinGreen—EggshellofLushiChickenpresentedfirstdecreasesandthenincreasesandthendecreasesasthefluctuation．Thebiliverdincontentintheeggshelli
6、ntwenty—secondweekswashigherthanthatintwenty—fifthweeks：andthedifferencesbetweenthenweresignificant．Thebiliverdincontentintheeggshellintwenty—eighthweekswashigherthanthatintwenty—fifthweeks．a(chǎn)ndthediffere：ricesbetweenthenweresignificant·(2)Inthe22．31weekageeggqualityoftheGreen—EggshellofLushi
7、Chickenincreasedastheweekage．Yolkweightincreasedgradually，andtherewasasignificantdifferencebetweenthem．(3)Inthe22．31weekagethevillusheightoftheeggshellinGreen—EggshellofLushiChickenincreasedgradually,andthevillusheightintwenty。secondweekshadasignif
