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1、萬方數(shù)據(jù)英文摘要EffectofmTORsignalingpathwayonischemia—reperfusioninjuryinratsColledgeofPharmaceuticalScience，ZhejiangUniversityPharmacyCandidateforMasterSupervisorAbstractHuadanZhangProf．LinghuiZengobjective：CerebralischemiaiSaseriousdiseasewhichlcadstoextensivecerebraldamageandseriousneurologic
2、aldeficits．Tillnow，tissueplasminogenactivator(tPA)remainstheonlyapproveddrugproventOimproveclinicaloutcomew翊、ischemicstroke．However．theuseoftPAiSlimitedbecauseofanincreasedriskofhemorrhagefewhourspost—stroke．Therefore，itisurgentforinvestigatingnewtherapeuticstrategiesforisehemicstroke。Mam
3、maliantargetofraparnycin(roTOR)isacriticalregulatorofcellgrowthandmetabolismthatisimplicatedinmanydiseases．Rapamycin，aninhibitorofmTOl乙wasdiscoveredtoantagonizetheanti—apoptoticsignalsandstimulateautophagytomaintainnormalcellmetabolism．Ithasalsodemonstratedeffectsinbehavioralandlearningin
4、theanimalmodeloftraumaticbraininjury．However,theeffectandmechanismofmTOR—mediatedprotectioninischemicstrokeisunclear．Inthepresentstudy，weexploredwhetherthemTORsignalingpathwayisinvolvedinischemia-reperfusioninjuryinSDrats．Methods：wecarriedoutmiddlecerebralarteryocclusion(MCAO)withsutureme
5、thodinSDratstomimictheprocessofhumancerebralischemia．neneurologicalcriterionofratsatdifferenttimepointsafterMCAOmodelingWasmonitored．ThechangesofthemTORsignalingpathwayindifferenttimepointandbrainareaweredetectedusingWesternBlotafterMCAOmodeling．TTCstainingwasusedtoanalyzetheinfarctsizebe
6、foreandaftermodeling．FJBstainingWascarriedouttomeasureapoptoticcells．CD68fluorescentstainingWasusedtomeasurethenumberofmicroglialcell．Results：mTORpathwaywasactivatedTV萬方數(shù)據(jù)浙江大學(xué)碩士學(xué)位論文英文摘要60minutesafterMCAOintheischemicpenumbra．Aswesetischemictimefor1handobservedmTORpathwayatdifferentinterva
7、lsofreperfusion，wefoundthatactivationofmTORpathwaykeptathigherlevelfrom6hhoursafterperfusion．Long-termmonitoringafterischemiashowedthatmTORsignalingpathwaystillkeptathighlevelfrom2weeks．Pre·administrationandpost—administrationofrapamycinsignificantlyimprovedneurolog
