壯筋續(xù)骨湯對骨折大鼠血清bfgf和igf-1含量的影響和意義

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1、壯筋續(xù)骨湯對骨折大鼠血清bFGF和IGF．1含量的影響和意義摘要目的：研究壯筋續(xù)骨湯在大鼠股骨骨折愈合過程中bFGF和IGF．1的表達(dá)變化情況，以及壯筋續(xù)骨湯對骨折后愈合的促進(jìn)作用。方法：成年的雄性健康Wistar大鼠共60只，用電鋸在股骨的中段部分切斷股骨，制備大鼠的股骨骨折模型，造模成功后壯筋續(xù)骨湯灌胃治療。中藥治療后的不同時間觀察大鼠的肢體活動情況，X線觀察骨折愈合的情況，HE觀察骨折愈合的病理變化。ELISA檢測血清中bFGF和IGF．1水平。結(jié)果：股骨骨折后局部腫脹，活動受限。x射線顯示治療7天后，局部腫脹消失，14天，纖維性骨

2、痂形成，21天，骨折線模糊，28天骨折線消失。模型組大鼠血清bFGF和IGF-1水平均顯著高于假手術(shù)組，P

3、essionofCGRPandIGF一1inRatsABSTRACTobjective：ToresearchtheeffectofZhuangJinXuGuDecoctioninpromotingbFGFandIGF-1expressioninfemoralfracturecallus，andthentodiscussthefracturehealingfunctionofZhuangJinXuGuDecoction．Methods：Totalofseventy-twoadultandhealthymaleWistarrats．which

4、weremadefracturemodelwithelectricsawthatwerecutthroughthemiddlepointoffemur．ThemodelgroupwastreatedbyinfusingZhuangJinXuGuDecoctionthroughstomach．ThentoObservethebodyactivitiesindifferentdays，thefracturehealingsituationwasobservedthroughtthewayofX-rayfilm，inthelastthepath

5、ologicalchangewasdetectedbyHematoxylin-Eosinstaining(HE)．TheserulTllevelsofbFGFandIGF-1weregetbythewayofenzyme-linkimmunobsorbantassay(ELISA)．Results：localswellingandrestrictedmovementafterFemoralfi'actures．7claysaftertreatment，localswellingdisappeared，14days，fibrouscallu

6、sformation,21days，fracturelinesblurred,28daysfracturelinedisappearedfromthetestofXray．Inthemodelgroup，theexpressionsofbFGFandIGF-1Washigherthantheratsinsham—operatedgroup(P<0．05)．TheserumlevelsofbFGFandIGF一1intreatmentgroupwerehigherthanthoseinmodelgroup(P<0．05)．21daysaft

7、ertreatment,theexpressionofserumbFGFandIGF—IWastoaspike．Conclusion：ZhuangJinXuGuDecoctionhavetheeffecttopromotingfemoralfracturecallusofrats,whichmightplayapartinincreasingtheexpressionsofbFGFandIGF一1Postgraduatestudent：PanYue—xingDirectedbyProfessor：GuoYun-liangKeywords：

8、ZhuangJinXuGuDecoction；Fracture；bFGF；IGF·1；Wistarrats目錄引言???????????????????????????????????????