資源描述：
《運(yùn)動對肥胖大鼠脂肪細(xì)胞脂聯(lián)素mrna表達(dá)的影響new》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫。
1、第26卷第2期西安體育學(xué)院學(xué)報(bào)Vol126No122009年3月JOURNALOFXI′ANPHYSICALEDUCATIONUNIVERSITYMar12009運(yùn)動人體科學(xué)文章編號:10012747Ⅹ(2009)0220206204文獻(xiàn)標(biāo)識碼:A中圖分類號:G80417X運(yùn)動對肥胖大鼠脂肪細(xì)胞脂聯(lián)素mRNA表達(dá)的影響1223李世成,邵雄杰,吳蘋,劉軍(1.湛江師范學(xué)院體育科學(xué)學(xué)院,廣東湛江524048;2.中山大學(xué)第三醫(yī)院,廣州510630;3.解放軍422醫(yī)院,廣東湛江524000)摘要:目的探討運(yùn)動對脂肪細(xì)胞脂聯(lián)素mRNA表達(dá)的影響,從脂肪細(xì)胞內(nèi)分泌的角度研究肥胖的機(jī)制。方法斷
2、乳5天SD健康雄性幼鼠40只,體重75-85g隨機(jī)分組為普通飼料喂養(yǎng)組和高脂飼料喂養(yǎng)組,高脂飼料喂養(yǎng)組在3周后篩選出肥胖鼠,并隨機(jī)分組為不運(yùn)動對照組和運(yùn)動干預(yù)組。運(yùn)動大鼠進(jìn)行游泳運(yùn)動,運(yùn)動時(shí)間第1周為30min/d、第2周為60min/d(運(yùn)動30min休息5min)、第3周為90min/d(運(yùn)動30min休息5min)、第4周為120min/d(運(yùn)動30min休息5min),每周運(yùn)動6d,周日休息,共4w。實(shí)驗(yàn)后處死所有大鼠,取睪丸脂肪墊和腹膜后脂肪組織測試脂聯(lián)素mRNA表達(dá)水平。結(jié)果肥胖大鼠運(yùn)動4周后血清脂聯(lián)素濃度增加了51.81%(P<0.05),睪丸脂肪墊和腹膜后脂肪組織脂聯(lián)
3、素mRNA表達(dá)水平分別增加86.36%(P<0.05)和94.74%(P<0.05)。結(jié)論運(yùn)動明顯增高血清脂聯(lián)素水平和內(nèi)臟脂肪組織脂聯(lián)素mRNA的表達(dá)。關(guān)鍵詞:脂聯(lián)素mRNA;運(yùn)動;脂肪細(xì)胞;肥胖大鼠EffectsofAerobicExerciseonAdiponectinmRNAExpressioninAdipocyteofObeseRats1223LIShi2cheng,SHAOXiong2jie,WUPing,LIUjun(1.SchoolofPhysicalEducation,ZhanjiangNormalCollege,Zhanjiang524048,China;2.The
4、thirdAffiliatedHospitalofSunYat-senUniversity,Guangzhou510630,China;3.ThePLA422Hospital,Zhanjiang524005,China)Abstract:ObjectiveTostudytheeffectsofexerciseonexpressionofadiponectinmRNAinadipocyte,andthemechanismthatexer2ciseintervenedinobesitytobeelucidated.Methods40maleratswhoseweightwasbetwee
5、n75gand85gwereweanedforfivedaysanddividedintonormaldietandhighfatdietgroupsrandomly.After3weeks,Obeseratswerechoosedanddividedintocontrolgroupandexperimentgrouprandomly.Theratsinexperimentgroupweredealedwithswimimgtrainingfor4weeks,exercisedsixdaysperweekandrestedoneday.Exercisetimewas30minperd
6、ayinthefistweek,60minperweekinthesecondweek,90minperweekinthethirdweekand120minperweekinthelastweek.Fromsecondweek,Ratsswamfor30minandbreaked30min.After4weeks,allratswerekilledandthelevelsofadiponectinmRNAexpressionweremeasuredintestisfadpadandretroperitonealfatpadofrat.ResultsAsObeseratsexerci
7、sedin4weeks,thelevelofserumadiponectinwasincreased51.81%(P<0.05).andtheexpressionofadiponectinmRNAincreased86.36%(P<0.05)intestisfadpadandincreased94.74%(P<0.05)inretroperitonealfatpad.ConclusionsThelevelofserumadiponectinincrease
