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《不同訓(xùn)練強(qiáng)度對(duì)大鼠心肌線粒體呼吸鏈功能及自由基代謝的影響》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、EffectsofDifferentTrainingIntensityonFunctionsofRespiratoryChainandFreeRadicalMetabolisminMyocardialMitochondriaofRatsHuangCaiyunNorthwestNormalUniversity西北師范大學(xué)研究生學(xué)位論文作者信息不同訓(xùn)練強(qiáng)度對(duì)大鼠心肌線粒體呼吸鏈功能及論文題目自由基代謝的影響姓名黃彩云學(xué)號(hào)2010210706專業(yè)名稱運(yùn)動(dòng)人體科學(xué)答辯日期2013年5月聯(lián)系電話E_mail通信地址(郵編):備注:
2、目錄摘要..............................................................IAbstract..........................................................IV1文獻(xiàn)綜述..........................................................11.1運(yùn)動(dòng)對(duì)線粒體呼吸鏈功能的影響................................11.2運(yùn)動(dòng)對(duì)自由基代謝的影響
3、......................................32選題依據(jù)..........................................................83實(shí)驗(yàn)對(duì)象與方法...................................................103.1實(shí)驗(yàn)對(duì)象及分組.............................................103.1.1實(shí)驗(yàn)動(dòng)物.................................
4、............103.1.2適應(yīng)性訓(xùn)練及動(dòng)物篩選.................................103.1.3動(dòng)物分組及訓(xùn)練安排...................................103.2實(shí)驗(yàn)取材及線粒體制備.......................................113.2.1心臟取材.............................................113.2.2心肌線粒體的提取.........................
5、............113.3測(cè)試指標(biāo)及方法.............................................113.3.1線粒體蛋白質(zhì)含量的測(cè)定...............................113.3.2NADH、DCPIP、細(xì)胞色素C消光系數(shù)的測(cè)定................123.3.3線粒體呼吸鏈酶復(fù)合物活性的測(cè)定.......................133.4自由基代謝相關(guān)指標(biāo)的測(cè)定...................................14
6、3.4.1超氧化物岐化酶(SOD,superoxidedismutase)活性測(cè)定..143.4.2谷胱甘肽過(guò)氧化物酶(GSH-PX)活性測(cè)定.................153.4.3過(guò)氧化氫酶(CAT)的活性..............................153.4.4丙二醛含量(MDA)測(cè)定................................163.5藥品和試劑.................................................163.6實(shí)驗(yàn)儀器......
7、.............................................163.7數(shù)據(jù)統(tǒng)計(jì)與處理.............................................173.8實(shí)驗(yàn)技術(shù)路線...............................................174實(shí)驗(yàn)結(jié)果.........................................................184.1不同訓(xùn)練強(qiáng)度對(duì)大鼠體重的影響....................
8、...........184.2測(cè)試樣本蛋白質(zhì)的含量.......................................184.3不同訓(xùn)練強(qiáng)度對(duì)大鼠心肌線粒體呼吸鏈酶復(fù)合物Ⅰ~Ⅳ活性的影響.194.3.1不同訓(xùn)練強(qiáng)度對(duì)大鼠心肌線粒體呼吸鏈酶復(fù)合物Ⅰ活性的影響.194.3.2不同訓(xùn)練強(qiáng)度對(duì)大鼠心肌線粒體呼吸鏈