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1、分類號(hào):密級(jí):HUAZHONGAGRICULTURALUNIVERSITY碩士學(xué)位論文MASTER’SDEGREEDISSERTATION水稻和陸稻分化相關(guān)基因E1899的功能研究THEFUNCTIONOFLOWLANDRICEANDUPLANDRICEDIFFERENTIATIONRELATEDGENEE1899研究生:李興濤CANDIDATE:LIXINGTAO學(xué)號(hào):2016301120069STUDENTNO.:專業(yè):農(nóng)業(yè)碩士MAJOR:MasterofAgriculture導(dǎo)師:羅利軍教授SUPERVISOR:PROFESSORL
2、UOLIJUN中國(guó)武漢WUHAN,CHINA二○一八年六月JUNE,2018華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文水稻和陸稻分化相關(guān)基因E1899的功能研究ThefunctionoflowlandriceanduplandricedifferentiationrelatedgeneE1899研究生:李興濤學(xué)號(hào):2016301120069指導(dǎo)教師:羅利軍教授學(xué)位類型:農(nóng)業(yè)碩士領(lǐng)域:作物華中農(nóng)業(yè)大學(xué)中國(guó)·武漢HuazhongAgruiculturalUniversityWuhan,China分類號(hào):密級(jí):水稻和陸稻分化相關(guān)基因E1899的功能初步研究目錄摘
3、要.............................................................i關(guān)鍵詞.............................................................ⅱABSTRACT..........................................................iiiKeywords..........................................................ⅳ縮略詞表....
4、.......................................................ⅴ1前言..............................................................11.1干旱脅迫對(duì)水稻的影響.........................................11.2水稻抗旱性研究進(jìn)展...........................................21.3水陸稻分化及陸稻資源的利用.......................
5、............51.4DUF538家族研究概況..........................................61.5葉綠素與水稻抗旱.............................................81.6酵母雙雜交原理及在水稻中應(yīng)用.................................81.7本研究的目的和意義..........................................102材料與方法...............................
6、.......................112.1試驗(yàn)材料..................................................112.2實(shí)驗(yàn)方法..................................................122.2.1水稻材料培育方法......................................122.2.2水稻抗旱性鑒定........................................122.2.3水稻表達(dá)量分析..........
7、..............................122.2.4E1899蛋白酶活性實(shí)驗(yàn)..................................132.2.5葉綠素含量和葉綠素?zé)晒鈪?shù)測(cè)定.......................142.2.6本研究一般載體構(gòu)建....................................142.2.7酵母雙雜交方法........................................152.2.8蛋白互作的煙草體內(nèi)驗(yàn)證....................
8、............172.2.9亞細(xì)胞定位............................................172.2.10生物信息學(xué)分析.............