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《達(dá)托霉素高產(chǎn)菌株選育及發(fā)酵條件優(yōu)化》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、天津大學(xué)碩士學(xué)位論文達(dá)托霉素高產(chǎn)菌株選育及發(fā)酵條件優(yōu)化姓名：范晶華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化工指導(dǎo)教師：聞建平20070101ABSTRACTDaptomycinisanewlipopeptideantibioticwhichcontainsaringwithteenaminoacidsandthreeaminoacidswitha10．carbondecanoicacidattachedtothetcrminalL-tryptophan．DaptomycinisproducedbyStreptomycesroseos
2、porus，anditsactivitylimitedtogram-positiveorganisms，especiallytheantibiotic-resistantbacteriasuchasStaphvlococcusaureus(MRSA)，vancomycin-resistantenterococciWry)．DaptomycinwasapprovedbyFDAin2003，anditwasusedinskinandsofttissueinfection．However，theinternalresearcho
3、fdaptomycinproductionisrare，sotheapplicationofdaptomycinislimitedseverely。Therefore，itisofgreatimportancetoenhancetheresearchanddevelopmentofdaptomycinproductionprocess．Inthiswork，thehighproductivitystrainWasselectedfordaptomycinproductionandthemediumcompositionan
4、dfermentationconditionswercoptimized．Themajorresearchcontentsandresultsareasfollows：，StreptomycesroseosporusLD-1wasused黼anoriginalstrain。Aftertreatedwithdiethylsulfateand1aserirradiationinseries，amutantstrainLD．54Wasscreenedoutbyusingthemediumwithselectivepressure
5、ofstreptomycin．Underthefermentationconditionwithoutbeingoptimized，theproductionofdaptomycinwas34．29m醣，whichwas1．94timeshi曲el"thanthatoftheparentalstrainLD一1二Thestrain始geneticallystable．Thebatchfermentationofdaptomycinproduction、)I，iththestrainLC一54inshakeflaskWaSs
6、tudied。TheoptimalseedageandthefermentationmediumfordaptomycinproductionweredesignedbyusingSAS．Also，theeffectsofcultivationconditionssuchasinoculum，rotatespeed，initialpHvalue，andculturemediumondaptomycinproductionbyStreptomycesroseosporu$wereevaluated．Undertheoptim
7、umconditions，strainLC一54couldproducedaptomycin54．5mg／ebyshaking—flaskbatchfermentation。whichwas58．9％higherthanthatofinitialconditions。TheeffectofprecursorondaptomycinproductionWasstudied．A7．5LbioreactorWasusedtostudythedaptomycinfermentation，theproductionofdaptomy
8、cinreached126．6mga．。intheoptimizedoperatingcondition，whichWas133％higherthanthatinshaking-flaskfermentation。AlogisticmodelWasadoptedtosimulatetheexperime
