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《融合基因FcyFur5氟胞嘧啶系統(tǒng)對人卵巢癌細(xì)胞HO8910裸鼠皮下移植瘤生長抑制作用的初步研究+姜黃素對人卵巢癌細(xì)胞株HO8910生長抑制作用的實(shí)驗(yàn)研究》由會員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、南京隧科大學(xué)碩士學(xué)位論文融合基因Fcy::Fur/5一氟胞嘧嚏系統(tǒng)對人擘目巢癌細(xì)胞株HO-8910裸鼠瘦下移植瘤生長抑制作用的初步研究中文摘要愛的:探討融合基因Fcy::Fur/5-氟胞嘧啶系統(tǒng)對人卵巢癌細(xì)胞株HO-8910細(xì)胞裸鼠皮下移植瘤生長的影響及英作用機(jī)鑭。方法:1。裸鼠皮下分別滋射卵巢癌細(xì)胞株tt0-8910細(xì)胞和穩(wěn)定表達(dá)融合基因Fcy::Fur的HO-8910細(xì)胞(HO一8910-Fcy::Fur),構(gòu)建裸鼠卵巢癌疫下移植瘤模型。2.將成瘤后的裸鼠隨機(jī)分為對照組、重組腐粒LZRS-Fcy::F
2、ur/5-FC治療紐爭轉(zhuǎn)基因HO一8910-Fcy::Fur/5一FC治療組。對照級給予黢靛注射PBS;重組質(zhì)粒組于治療第一天瘤體內(nèi)注射LZRS-Fcy::Fur質(zhì)粒,黢碹予羲傣藥物5一K治療;轉(zhuǎn)基霹紐疆腔注射5-FC。治療舞始露每5天測瘸長、寬,計(jì)算瘤體積。3。治療20天后處死裸鼠,測癌長、寬,瘤體稱重,計(jì)算瘤體積、抑瘤率,瘤組織標(biāo)本分別進(jìn)行HE染色病理檢查和流式細(xì)胞權(quán)檢測。南索鹱科大學(xué)碩士學(xué)巍論文結(jié)果:轉(zhuǎn)基因治療紐從治療第10天起對卵巢癌裸鼠皮下移植瘤的生長具有明顯抑制作用,重組質(zhì)粒治療組則未見有明顯
3、作用,兩組抑摘率分另《為12.96甏乖72。53%,病緩和流式耋霹施儀檢測瘤緩織標(biāo)本提示治療后的腫瘤細(xì)胞發(fā)生了凋亡及S期阻滯。結(jié)論:融合基霞Fcy::Fur/5一氯融嘧嚏系統(tǒng)對人努巢癌緞Fe_AgIt0-8910裸鼠皮下移植瘤生長具有明顯的抑制作用,其機(jī)制可能與引起腫瘤細(xì)胞凋亡和細(xì)胞周期的改變有關(guān)。體外轉(zhuǎn)染效果較體內(nèi)轉(zhuǎn)染效果明渡?!娟P(guān)鍵讕1融合型鑫殺基因Fcy::Fur辯巢癌裸鼠南索暇辯大學(xué)碩士學(xué)位論文PreliminaryStudyonInhibitoryEffectofFusionSuicideGen
4、eFcy::Fur/5-FiuorocytosineTherapiesontheSubcutaneousTumorsTransplantedWithHumanOvarianCancerCdlLineHO-8910inNudeMiceABSTRACTObjective:Thepurposeistoinvestigatetheinhibitoryef瓷ctoffusionsuicidegeneFcy::Fur/5一Fluorocytosinetherapiesonthegrowthofthesubcutane
5、oustumorstransplantedwithhumanovariancancercelllineH@8910innudemiceanditsmechanisms.Methods:1.Buildnudemicemodel"nudemiceweresubcutaneouslyimplantedwithHO一8910cellsorHO一8910一Fey::Ftlfcells。2.WhenthediameterofthetumorswasaboutlOmm,t11eyweredividedrandomlyi
6、ntothreegroups.ControlgroupwasadministeredPBSbyintraperitonealinjection;plasmidgroupreceivedintratumoralinjectionsofplasmidLZRS—Fcy::Fur,plasmidgroupandHO一8910-Fcy::Furgroupwerebothadministered5-FCbyintraperitonealinjection.Thenthevolumeofthetumorswascalc
7、ulatedevery5days3.Thenudemicewerekilledonday20,theweightofimplantedtumors南京暇科大學(xué)碩士學(xué)短論文andthetumorinhibitionrateswerecalculated,thesamplesofimplantedtumorswerestudiedwithHEstainingandexaminedwithFCM.Results:Comparedwithcontrolgroup,thegrowthoftumorsinHO一891
8、0-Fey::Furgroupwassignificantlysuppressed.From10daysafterthethempies,therewassignificantdifferencebetweenHO-8910一Fcy::Furgroupandcontrolgroup,whiletherewasnotsignificantdifferencebetweenplasmidgroupandcontrolgroup,t