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《小麥根系分泌物對(duì)黃瓜生長(zhǎng)及土壤真菌群落結(jié)構(gòu)的影響_吳鳳芝》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、應(yīng)用生態(tài)學(xué)報(bào)2014年10月第25卷第10期ChineseJournalofAppliedEcology，Oct．2014，25(10):2861－2867DOI:10.13287/j.1001-9332.2014.0156小麥根系分泌物對(duì)黃瓜生長(zhǎng)及*土壤真菌群落結(jié)構(gòu)的影響＊＊吳鳳芝李敏曹鵬馬亞飛王麗麗(東北農(nóng)業(yè)大學(xué)園藝學(xué)院，哈爾濱150030)摘要以黃瓜為受體，以不同化感效應(yīng)(促進(jìn)/抑制)小麥品種為供體，采用PCＲ-DGGE技術(shù)，研究了小麥根系分泌物及伴生小麥對(duì)黃瓜生長(zhǎng)及土壤真菌群落結(jié)構(gòu)的影響．結(jié)果表明:在處理第6天和第12天，化感促進(jìn)
2、效應(yīng)小麥根系分泌物分別顯著提高了黃瓜幼苗株高和莖粗;在處理第18天，化感促進(jìn)和抑制效應(yīng)小麥根系分泌物均顯著提高了黃瓜幼苗株高;在處理第6天，不同化感效應(yīng)小麥根系分泌物均顯著降低了黃瓜幼苗根際土壤真菌群落條帶數(shù)、Shannon指數(shù)及均勻度指數(shù)，有苗對(duì)照(W)顯著高于無(wú)苗對(duì)照(Wn);在處理第18天，各處理的真菌群落結(jié)構(gòu)條帶數(shù)、Shannon指數(shù)及均勻度指數(shù)均顯著高于無(wú)苗對(duì)照(Wn)．伴生化感抑制效應(yīng)小麥顯著降低了黃瓜根際土壤真菌群落Shannon指數(shù)和均勻度指數(shù)，說(shuō)明小麥根系分泌物及伴生小麥改變了土壤真菌群落結(jié)構(gòu)．DGGE圖譜及其主成分分析
3、結(jié)果表明，伴生不同化感效應(yīng)小麥對(duì)土壤真菌群落結(jié)構(gòu)影響較大．關(guān)鍵詞小麥根系分泌物黃瓜伴生真菌群落結(jié)構(gòu)文章編號(hào)1001－9332(2014)10－2861－07中圖分類(lèi)號(hào)Q5文獻(xiàn)標(biāo)識(shí)碼AEffectsofwheatrootexudatesoncucumbergrowthandsoilfungalcommunitystructure．WUFeng-zhi，LIMin，CAOPeng，MAYa-fei，WANGLi-li(CollegeofHorticulture，NortheastAgricul-turalUniversity，Harbin15
4、0030，China)．-Chin．J．Appl．Ecol．，2014，25(10):2861－2867．Abstract:Withwheatasthedonorplantandcucumberasthereceptorplant，thisstudyinvestiga-tedtheeffectsofrootexudatesfromwheatcultivarswithdifferentallelopathicpotentials(positiveornegative)andcompanioncroppingwithwheatonsoilfu
5、ngalcommunitystructurebyPCＲ-DGGEmethodandcucumbergrowth．Ｒesultsshowedthatthewheatrootexudateswithpositiveallelopathicpotentialincreasedheightandstemdiameterofcucumberseedlingssignificantly，comparedtothecontrolseedlings(W)after6daysand12daystreatment，respectively．Also，whea
6、trootexudateswithbothpositiveandnegativeallelopathicpotentialincreasedtheseedlingheightofcucumbersig-nificantlyafter18daystreatment．Thewheatrootexudateswithdifferentallelopathicpotentialsde-creasedthebandnumber，Shannonandevennessindicesofsoilfungalcommunitysignificantlyin
7、cucumberseedlingrhizosphere，andthoseinthesoilwiththecontrolseedlings(W)werealsosig-nificantlyhigherthanthatinthecontrolsoilwithoutseedlings(Wn)after6daystreatment．Thebandnumber，Shannonandevennessindicesinallthetreatmentsweresignificantlyhigherthanthoseinthecontrolsoilwith
8、outseedlings(Wn)after18daystreatment．Companioncroppingwithnegativeallelopathicpotentialwheatdecr
