測(cè)序通用引物列表

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1、測(cè)序通用引物列表測(cè)序通用引物列表載體名稱上游引物（5'primer）下游引物（3'primer）P3*FLAG-CMVCMV-30(825-854)CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C)pAc5-5BGHrevpAcG2TpGEX-5notavailablepACTT7EEVT3/EBVrevpACT2GAL4ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site)PBRforBamPBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pA

2、CYCDuet-1(MCSII)DuetUp2T7-TerminatorpAdEasy-1notavailablenotavailablepAdTrack-CMVnotavailablenotavailablepAS2-1GAL4Bdfor/P24990pGAL4-BDrv/P31430pB42ADpB42ADFPB42ADRpBABEpBMN_5'pBacPAK8BAC1BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5'pBBR1MCST3/M13revT7/M13forpBD-GAL4pBD-GAL4-FPBD-GAL4-RpBI12135SNOS

3、pBINDGAL4-BdforT3,EBVrevpBK-CMVM13F/T7M13R/T3pBluescriptIIKS(+/-)T3/M13revT7/M13for（優(yōu)先用M13F）pBluescriptIISK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site)PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites)pBRforEcopBRrevHindpBR325(B

4、amHI-site)PBRforBamPBRrevBampBR329(BamHI-site)PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3'BDpBridge(MCSII)notavailablenotavailablepBS-TIIT3/M13revT7/M13forpBS（AMP）T7T3pBSRT3T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1αFwdBGH（第1頁(yè)共10頁(yè)）測(cè)序通用引物列表pBudCE4.1/lacZ/CATCMV-Profor

5、,T7c-mycrev,EBVrevpBV220pBV220FPBV220RpCAL-cT7/Tac-ProforT7-TerminatorpCAL-nT7/Tac-ProforT7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5'(ECORI)/M13RpCAMBIA1301-3'(HindIII)pCAMBIA1304pCAMBIA1301-5'(ECORI)pCAMBIA1301-3'(HindIII)pCAMBIA2300M13R(-48)M13F(-47)/M13F(-49)pCANTB5ES1S6pCAT3-enhancerRVP3

6、此載體無(wú)反向引物pCDFDuet-1(MCSI)ACYCDuetUP1PrimerDuetDOWN1pCDFDuet-1(MCSII)DuetUP2T7TERpcDNA1.1CMV-Profor,T7pCDM8-3pcDNA2.1M13for/T7M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGHrev/SP6pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGHrevpcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGHrevpcDNA3.1/myc-His(_A,_B,_C)T7/CMV-Profor

7、BGHrev/c-mycrevpcDNA3.1/V5-His(_A,_B,_C)T7/CMV-ProforBGHrev/V5revpcDNA3.1/Hygro(+,-)T7/CMV-ProforBGHrev/V5revpcDNA3.1/Zeo(+.-)T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C)XpressforBGHrevpcDNA4/HisMax-TOPOXpressforBGHrevpcDNA4/H