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1、BG11液體培養(yǎng)基BG11液體培養(yǎng)基就是：Stock1定容100mL檸檬酸0.3g檸檬酸鐵胺0.3gEDTANa20.05gStock2定容1000mLNaNO330gK2HPO40.78gMgSO4·7H2O1.5gStock3定容100mLCaCl2·2H2O1.9gStock4定容100mLNa2CO32gStock5定容1000mLH3BO32.86gMnCl2·4H2O1.81gZnSO4·7H2O0.222gNa2MnO4·2H2O0.391gCuSO4·5H2O0.079gCo(NO3)2·6H2O0.049gStock1取用2mLSto
2、ck2取用20mLStock3取用2mLStock4取用1mLStock5取用1mL總定容1000mLD1(Diatommedium)?Component????????????Amount????????StockSolution????(1)NaNO3??????????????????1mL/L????????????12g/100mldH2O?????????(2)K2HPO4?????????????????1mL/L????????????4g/100mLdH2O????????(3)MgSO4·7H2O?????????1mL/L????
3、????????7g/100mLdH2O????????(4)CaCl2·2H2O??????????1mL/L????????????2g/100mLdH2O??????(5)KH2PO4????????????????1mL/L????????????8g/100mLdH2O???????(6)Na2SiO3.9H2O????????1mL/L???????????10g/100mldH2O(7)MnSO4?????????????????0.1mL/L????????????0.2g/100mldH2O(8)檸檬酸鐵???????????????
4、?1mL/L?????????????0.5g/100mldH2O(9)A5（Tracementalsolution）?1ml/L?????????????????H3BO3?????????????????????2.86g/LdH2O?????????MnCl2·4H2O??????????????1.86g/LdH2O?????????ZnSO4·7H2O?????????????0.22g/LdH2O?????????Na2MoO4.2H2O????????????0.39g/LdH2O?????????CuSO4.5H2O??????????
5、????0.08g/LdH2O?????????Co(NO3)2.6H2O???????????0.05g/LdH2O(10)土壤提取液??????????????20mL/L?土壤提取液配制方法:取花園土未施過肥200g置于燒杯或三角瓶中，加入蒸餾水1000毫升，瓶口用透氣塞封口，在水浴中沸水加熱3小時，冷卻，沉淀24小時，此過程連續(xù)進行3次，然后過濾，取上清液，于高壓滅菌鍋中滅菌后于4℃冰箱中保存?zhèn)溆谩?f/2?mediumComponent??????????????????????????Amount??????????(1)?NaNO3???
6、???????????????????75mg/L(2)?NaH2PO4·2H2O?????????0.6mg/L(3)?VitaminB12????????????????0.5μg/L(4)??Biotin???????????????????????0.5μg/L(5)?ThiamineHCl??????????????100μg/L(6)?Na2SiO3·9H2O??????????10mg/L(7)??f/2metals?????????????????1mL/L???????Na2EDTA·2H2O?????4.4g???????FeCl3
7、·6H2O????????3.16g???????CoSO4·7H2O???????0.012g???????ZnSO4·7H2O???????0.021g???????MnCl2·4H2O?????????0.18g???????CuSO4·5H2O???????0.007g??????Na2MoO4·2H2O??????0.007g???????Distilledwater????????1000mL(8)???Seawater????????????????????????1000mL
