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1、STUDYoNMICROPROPAGATIoNOFWALNUTABSTRACTWalnut(JuglansregiaL．)wasfromwestEuropeandAsiaOriginally，ourcountryisoneofwalnutoriginalhabitats【‘’“．Thewalnutisthefineeconomicaltreebecauseofihefruitandlignumuses．a(chǎn)soneofthefourdriedfruit,itwascultivatedwidespreadintheworldbecauseofitsecono
2、my，ecologyandsocialefficieney，buttherearemanydifficultiesinextendingoftheimprovedseedwalnut．Duringthepropagationofwalnuttissues，thechosenexplantsappearstohighpollutionrate，thewalnutbudstumingbrownserious，lowreproducibilityofthenextgenerations，theseedlingtakinglittleandweakrootsas
3、wellasthemicro-graftsasthegraftmethodsleadingtothelowsurvivalrateandsoon,thispaperhasstudiedthatdifferentwalnuttissuesastheexplants，thefactorsinducingthewalnutsomaticeellbecomeembryoandmicrograftingtechnology．Testresultasfollows：1．Studyontissuecultureofwalnut1．1n圮mostsuitablecult
4、uremediuminprimarycultivateiSDKW+6-BAI．0mg／L+IBA0．11119門L+Ve5．0me,／L+agar6．0egL+suorose309／L．1．2SelectingmaterialfromthemiddleofMaytothefirsttendaysofJune，thepollutionratesarelowerthan5％，reaching3．1％；SelectingmaterialinMayandinAugustcouldreducethebrowningrates．a(chǎn)ndreachthelowestin
5、August,is25．64％．1．3111ebrowningratesof8-week-oldseedlingsdirectlyfromseediSonly17．2％。one-year-oldis26．3％．butthegrown-uptreesreach48．5％1．4Theantioxidantandsorbenttestresultindicatedthat,Vc5mg／Lmaymakethebrowningrotestothelowest．is8．87％．1．5T1letestresultofeontinuativecultivateindic
6、atethat,themosteffectiveconcentrationof6．BAiS1．0mg／LandthecorrespondingconcentrationofIBAisbeBⅣecn0．01—0．05mg／L．n圮strongtubecultureseedlingstestresultindicatedthat,theDKWculturemediumattache0．25rag／L6．BAand0．03mg／LIBAiSthemostsuitableculturemediuminthistest；n圮suitabletransfercycl
7、eis25-30d；ThemostsuitablepHforthegrowthandthemultiplicationofthewalnuttesttubeseedlingis5．8．1．6111esuitableculturemediuminradicationisI／2DKW+IBA3．Omg／L+2．09／LAC+6．09／Lagar+30．Og／L$uerose，theradicationratescanachieve25．93％．a(chǎn)ndthepromotioneffectsinradicationofNAAislowefthanIBAobvio
8、usly．2．StudyOilmicrngraffingofwalnut2．1n
