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1、www.themegallery.comMultipleandHigh-ThroughputDropletReactionsviaCombinationofMicrosamplingTechniqueandMicrofluidicChipMembers:李娜馮雙霞徐秋燕劉婷婷聶懷軍ContentsBackgroundExperimentABSTRACTDiscussionABSTRACTMicrodropletso?eruniquecompartmentsforaccommodatingalargenumberofchemicalandbiological
2、reactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicro?uidicsisthedi?cultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicro?uidicchiptoperformdropletscreeningsandaggressivereactio
3、nwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoinc
4、reasethechipsizeandcomplexity.Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.AdvantagesOverthepastfewyears,droplet-basedmicro?uidicshasattractedincreasingattention.BackgroundDropletgenerat
5、ionformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformati
6、onmethods:InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamples
7、orthecoveringoil.Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchipThreegeneralstepsloadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintot
8、hecontrollerviaaPCstartthecontrol
