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《Lysine Acetylation Is a Highly Abundant and Evolutionarily Conserved Modification in Escherichia Coli》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、ResearchLysineAcetylationIsaHighlyAbundantandEvolutionarilyConservedModificationinEscherichiaColi*□SJunmeiZhang?§,RobertSprung?§?,JiminPei?,XiaohongTan**,SungchanKim??,HengZhu§§,Chuan-FaLiu**,NickV.Grishin?,andYingmingZhao???LysineacetylationanditsregulatoryenzymesareknownEmergingevidencesugg
2、estsdiversenon-nuclearrolesoftohavepivotalrolesinmammaliancellularphysiology.lysineacetylationanditsregulatoryenzymes,inadditiontoitsHowever,theextentandfunctionofthismodificationinwellrecognizedfunctionsinDNA-templatedprocesses.Forprokaryoticcellsremainlargelyunexplored,therebypre-example,Sirt
3、1modulatesdiversecellularprocesses,suchassentingahurdletofurtherfunctionalstudyofthismodi-fatmetabolism,insulinproduction,glucosehomeostasis,andficationinprokaryoticsystems.Herewereportthefirstcellsurvival,ofwhichsomearemediatedthroughnon-nuclearglobalscreeningoflysineacetylation,identifying138
4、proteins(6–8).Threemembersofthesirtuinfamily(Sirt3,modificationsitesin91proteinsfromEscherichiacoli.NoneoftheproteinshasbeenpreviouslyassociatedwithSirt4,andSirt5)arelocatedinthemitochondrion(9).Acti-thismodification.Amongtheidentifiedproteinsaretran-vationofSirt1,amammalianorthologofyeastSir2,
5、byscriptionalregulators,aswellasotherswithdiversefunc-resveratrolleadstodiversemetabolicchangesinanimalstions.Interestingly,morethan70%oftheacetylatedpro-(10).Therolesoflysineacetylationinmetabolismarefurtherteinsaremetabolicenzymesandtranslationregulators,exemplifiedbythefactsthatthemodificati
6、onispresentinsuggestinganintimatelinkofthismodificationtoenergymorethan20%ofmitochondrialproteinsandishighlymetabolism.Thenewdatasetsuggeststhatlysineacety-enrichedamongmetabolicenzymes(11–14).Althoughthelationcouldbeabundantinprokaryoticcells.Inaddition,inventoryandbiologicalfunctionsoflysinea
7、cetylationsub-theseresultsalsoimplythatfunctionsoflysineacetyla-stratesineukaryoticcellshavebeguntounfold,especiallytionbeyondregulationofgeneexpressionareevolution-arilyconservedfrombacteriatomammals.Furthermore,inhistoneproteins
